Gene Therapy Protocols

  • Jeffrey R. Morgan

Part of the Methods in Molecular Medicine book series (MIMM, volume 69)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Charles P. Lollo, Mariusz G. Banaszczyk, Patricia M. Mullen, Christopher C. Coffin, Dongpei Wu, Alison T. Carlo et al.
    Pages 1-13
  3. Catherine H. Wu, Cherie M. Walton, George Y. Wu
    Pages 15-23
  4. Assem G. Ziady, Pamela B. Davis
    Pages 25-48
  5. Jun-ichi Miyazaki, Hiroyuki Aihara
    Pages 49-62
  6. Yasufumi Kaneda
    Pages 63-72
  7. Yadi Tan, Mark Whitmore, Song Li, Peter Frederik, Leaf Huang
    Pages 73-81
  8. Klaus Schughart, Ulla B. Rasmussen
    Pages 83-94
  9. Ronald V. Abruzzese, Fiona C. MacLaughlin, Louis C. Smith, Jeffrey L. Nordstrom
    Pages 109-122
  10. Lina Prasmickaite, Anders Høgset, Kristian Berg
    Pages 123-135
  11. Chia-Feng Kuo, Jeng Hwan Wang, Ning-Sun Yang
    Pages 137-147
  12. Stelios T. Andreadis, Jeffrey R. Morgan
    Pages 161-172
  13. William P. Swaney, Enrico M. Novelli, Alfred B. Bahnson, John A. Barranger
    Pages 187-202
  14. Karen E. Hamoen, Gulsun Erdag, Jennifer L. Cusick, Hinne A. Rakhorst, Jeffrey R. Morgan
    Pages 203-217
  15. Courtney Powell, Janet Shansky, Michael Del Tatto, Herman H. Vandenburgh
    Pages 219-231
  16. R. Todd Reilly, Jean-Pascal H. Machiels, Leisha A. Emens, Elizabeth M. Jaffee
    Pages 233-257
  17. Antonia Follenzi, Luigi Naldini
    Pages 259-274

About this book


Significant advances in gene transfer technology and the completion of sequencing the human genome offer much renewed hope that gene therapy will provide novel approaches to the treatment of inherited and acquired diseases. In this fully updated and revised 2nd edition of the warmly received Gene Therapy Protocols, leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. The methods range from those in which new molecular conjugates show great promise for targeting targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. In addition, there are completely revised methods based on retroviruses and adenoviruses, as well as new and promising methods based on lentiviruses and adeno-associated viruses. The quantitative aspects of gene therapy are strongly emphasized, and the book reflects the many significant recent improvements in gene transfer efficiency, gene regulation, and vector production.
Up-to-date and highly practical, Gene Therapy Protocols, 2nd Edition, offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven step-by-step laboratory procedures that enable successful therapeutic application.

Editors and affiliations

  • Jeffrey R. Morgan
    • 1
  1. 1.Brown UniversityProvidence

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2002
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-723-6
  • Online ISBN 978-1-59259-141-1
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
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