Skip to main content
SpringerLink
Log in
Book cover

Gene Therapy Protocols pp 63–72Cite as

Viral Liposomes

Preparation and Use

  • Protocol

  • 736 Accesses

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 69))

Abstract

With the aim of developing successful human gene therapy, numerous viral and nonviral (synthetic) methods of gene transfer have been developed (1,2), each method having limitations as well as advantages. To develop in vivo gene transfer vectors with high efficiency and low toxicity, several groups have attempted to overcome the limitations of one vector by combining them with the strengths of another.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Mulligan R. C. (1993) The basic science of gene therapy. Science 260, 926–932.

    Article  PubMed  CAS  Google Scholar 

  2. Ledley F. D. (1995) Non-viral gene therapy: the promise of genes as pharmaceutical products. Hum. Gene Ther. 6, 1129–1144.

    Article  PubMed  CAS  Google Scholar 

  3. Kaneda Y. (1998) Fusigenic Sendai-virus liposomes: a novel hybrid type lipo-some for gene therapy. Biogenic Amines 14, 553–572.

    CAS  Google Scholar 

  4. Kaneda Y., Saeki Y., Morishita R. (1999) Gene therapy using HVJ-liposomes; the best of both worlds. Mol. Med. Today 5, 298–303.

    Article  PubMed  CAS  Google Scholar 

  5. Dzau V. J., Mann M., Morishita R., and Kaneda Y. (1996) Fusigenic viral lipo-some for gene therapy in cardiovascular diseases. Proc. Natl. Acad. Sci. USA 93, 11421–11425.

    Article  PubMed  CAS  Google Scholar 

  6. Hirano T., Fujimoto J., Ueki T., et al. (1998) Persistent gene expression in rat liver in vivo by repetitive transfections using HVJ-liposome. Gene Ther. 5, 459–464.

    Article  PubMed  CAS  Google Scholar 

  7. Saeki Y., Matsumoto N., Nakano Y., et al. (1997) Development and characterization of cationic liposomes conjugated with HVJ (Sendai virus): reciprocal effect of cationic lipid for in vitro and in vivo gene transfer. Hum. Gene Ther. 8, 1965–1972.

    Article  Google Scholar 

  8. Chander R. and Schreier H. (1992) Artificial viral envelopes containing recom-binant human immunodeficiency virus (HIV) gp160. Life Sci. 50, 481–489.

    Article  PubMed  CAS  Google Scholar 

  9. Sawa Y., Kaneda Y., Bai H.-Z., et al. (1998) Efficient transfer of oligonucle-otides and plasmid DNA into the whole heart through the coronary artery. Gene Ther. 5, 1472–1480.

    Article  PubMed  CAS  Google Scholar 

  10. Goyal K. and Huang L. (1995) Gene therapy using DC-Chol liposomes. J. Lipo-some Res. 5,49–60.

    Article  CAS  Google Scholar 

  11. Yamada G., Nakamura S., Haraguchi R., et al. (1997) An efficient liposome-mediated gene transfer into the branchial arch, neural tube and the heart of chick embryos: a strategy to elucidate organogenesis. Cell. Mol. Biol. 43, 1165–1169.

    PubMed  CAS  Google Scholar 

  12. Yonemitsu Y., Kaneda Y., Muraishi A., et al. (1997) HVJ (Sendai virus)-cat-ionic liposomes: a novel and potentially effective liposome-mediated gene transfer technique to the delivery to the airway epithelium. Gene Ther. 4, 631–638.

    Article  PubMed  CAS  Google Scholar 

  13. Mabuchi E., Shimizu K., Miyao Y., et al. (1997) Gene delivery by HVJ-liposome in the experimental gene therapy of murine glioma. Gene Ther. 4,768–772.

    Article  PubMed  CAS  Google Scholar 

  14. Nishikawa T., Edelstein D., Du X.L., et al. (2000) Normalization of mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature 404, 787–790.

    Article  PubMed  CAS  Google Scholar 

  15. Kaneda Y., Iwai K., and Uchida T. (1989) Increased expression of DNA cointroduced with nuclear protein in adult rat liver. Science 243, 375–379.

    Article  PubMed  CAS  Google Scholar 

  16. Kaneda Y., Iwai K., and Uchida T. (1989) Introduction and expression of the human insulin gene in adult rat liver. J. Biol. Chem. 264, 12126–12129.

    PubMed  CAS  Google Scholar 

  17. Kato K., Nakanishi M., Kaneda Y., Uchida T., and Okada Y. (1991) Expression of hepatitis B virus surface antigen in adult rat liver. J. Biol. Chem. 266, 3361–3364.

    PubMed  CAS  Google Scholar 

  18. Tomita N., Morishita R., Higaki J., et al. (1996) In vivo gene transfer of insulin gene into neonatal rats by HVJ-liposome method resulted in sustained transgene expression. Gene Ther. 3,477–482.

    PubMed  CAS  Google Scholar 

  19. Tomita N., Higaki J., Morishita R., et al. (1992) Direct in vivo gene introduction into rat kidney. Biochem. Biophys. Res. Commun. 186, 129–134.

    Article  PubMed  CAS  Google Scholar 

  20. Isaka Y., Fujiwara Y., Ueda N., et al. (1993) Glomerulosclerosis induced by in vivo transfection with TGF-β or PDGF gene into rat kidney. J. Clin. Invest. 92, 2597–2601.

    Article  PubMed  CAS  Google Scholar 

  21. Morishita R., Gibbons G., Kaneda Y., Ogihara T., and Dzau V. (1993) Novel and effective gene transfer technique for study of vascular renin angiotensin system. J. Clin. Invest. 91, 2580–2585.

    Article  PubMed  CAS  Google Scholar 

  22. Okada Y. and Tadokoro J. (1962) Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich’s ascites tumor cells. Exp. Cell Res. 26, 98128.

    Google Scholar 

  23. Suzuki K, Nakashima H, Sawa Y, et al. (2000) Reconstituted fusion liposomes for gene transfer in vitro and in vivo. Gene Ther. Reg. 1, 65–77.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Gene Therapy Science, Graduate School of Medicine, Suita Osaka, Japan

    Yasufumi Kaneda

Authors
  1. Yasufumi Kaneda
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Brown University, Providence, RI

    Jeffrey R. Morgan

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Humana Press Inc.

About this protocol

Cite this protocol

Kaneda, Y. (2002). Viral Liposomes. In: Morgan, J.R. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 69. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-141-8:063

Download citation

  • DOI: https://doi.org/10.1385/1-59259-141-8:063

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-723-6

  • Online ISBN: 978-1-59259-141-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation