Abstract
Among the nonviral techniques for gene transfer in vivo, the direct injection of plasmid DNA into muscle is especially simple, inexpensive, and safe. However, applications of this method have been limited by the relatively low expression levels of the transferred gene. Recently, we investigated the applicability of in vivo electroporation for gene transfer into muscle, using plasmid DNA expressing a cytokine as the vector. The results demonstrated that gene transfer into muscle by electroporation in vivo is far more efficient than simple intramuscular DNA injection and provides a potential approach toward systemic delivery of cytokines, growth factors, and other serum proteins for human gene therapy.
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References
Wolff J. A., Malone R. W., Williams P., et al. (1990) Direct gene transfer into mouse muscle in vivo. Science 247, 1465–1468.
Wolff J. A., Ludtke J. J., Acsadi G., Williams P., and Jani A. (1992) Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle. Hum. Mol. Genet. 1, 363–369.
Davis H. L., Whalen R. G., and Demeneix B. A. (1993) Direct gene transfer into skeletal muscle in vivo: factors affecting efficiency of transfer and stability of expression. Hum. Gene Ther. 4, 151–159.
Davis H. L., Michel M.-L., and Whalen R. G. (1995) Use of plasmid DNA for direct gene transfer and immunization. Ann. N.Y. Acad. Sci. 772, 21–29.
Wolff J. A., Williams P., Acsadi G., Jiao S., Jani A., and Chong W. (1991) Conditions affecting direct gene transfer into rodent muscle in vivo. Biotechniques 11, 474–485.
Vitadello M., Schiaffino M. V., Picard A., Scarpa M., and Schiaffino S. (1994) Gene transfer in regenerating muscle. Hum. Gene Ther. 5, 11–18.
Wells D. J. (1993) Improved gene transfer by direct plasmid injection associated with regeneration in mouse skeletal muscle. FEBS Lett. 332, 179–182.
Tokui M., Takei I., Tashiro F., et al. (1997) Intramuscular injection of expression plasmid DNA is an effective means of long-term systemic delivery of interleukin-5. Biochem. Biophys. Res. Commun. 233, 527–531.
Tripathy S. K., Svensson E. C., Black H. B., et al. (1996) Long-term expression of erythropoietin in the systemic circulation of mice after intramuscular injection of a plasmid DNA vector. Proc. Natl. Acad. Sci. USA 93, 10876–10880.
Norman J. A., Hobart P., Manthorpe M., Felgner P., and Wheeler C. (1997) Development of improved vectors for DNA-based immunization and other gene therapy applications. Vaccine 15, 801–803.
Titomirov A. V., Sukharev S., and Kistanova E. (1991) In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA. Biochim. Biophys. Acta 1088, 131–134.
Muramatsu T., Mizutani Y., Ohmori Y., and Okumura J. (1997) Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochem. Biophys. Res. Commun. 230, 376–380.
Heller R., Jaroszeski M., Atkin A., et al. (1996) In vivo electroinjection and expression in rat liver. FEBS Lett. 389, 225–228.
Rols M.-P., Delteil C., Golzio M., et al. (1998) In vivo electrically mediated protein and gene transfer in murune melanoma. Nature Biotechnol. 16, 168–171.
Takatsu K. (1992) Interleukin-5. Curr. Opin. Immunol. 4, 299–306.
Aihara H. and Miyazaki J. (1998) Gene transfer into muscle by electroporation in vivo. Nat. Biotechnol. 16, 867–870.
Maruyama H., Sugawa M., Moriguchi Y., et al. (2000) Continuous erythropoietin delivery by muscle-targeted gene transfer using in vivo electroporation. Hum. Gene Ther. 11, 429–437.
Wolf H., Rols M. P., Boldt E., Neumann E., and Teissie J. (1994) Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophys. J. 66, 524–531.
Niwa H., Yamamura K., and Miyazaki J. (1991) Efficient selection for highexpression transfectants with a novel eukaryotic vector. Gene 108, 193–199.
Liang X., Hartikka J., Sukhu L., Manthorpe M., and Hobart P. (1996) Novel, high expressing and antibiotic-controlled plasmid vectors designed for use in gene therapy. Gene Ther. 3, 350–356.
Rizzuto G., Cappelletti M., Maione D., et al. (1999) Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation. Proc. Natl. Acad. Sci. USA 96, 6417–6422.
Mir L. M., Bureau M. F., Gehl J., et al. (1999) High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc. Natl. Acad. Sci. USA 96, 4262–4267.
Vicat J. M., Boisseau S., Jourdes P., et al. (2000) Muscle transfection by electroporation with high-voltage and short-pulse currents provides high-level and long-lasting gene expression. Hum. Gene Ther. 11, 909–916.
Harrison R. L., Byrne B. J., and Tung L. (1998) Electroporation-mediated gene transfer in cardiac tissue. FEBS Lett. 435, 1–5.
Couffinhal T., Kearney M., Sullivan A., Silver M., Tsurumi Y., and Isner J. M. (1997) Histochemical staining following LacZ gene transfer underestimates transfection efficiency. Hum. Gene Ther. 8, 929–934.
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Miyazaki, Ji., Aihara, H. (2002). Gene Transfer into Muscle by Electroporation In Vivo. In: Morgan, J.R. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 69. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-141-8:049
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DOI: https://doi.org/10.1385/1-59259-141-8:049
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-723-6
Online ISBN: 978-1-59259-141-1
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