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Gene Therapy Protocols pp 173–186Cite as

Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 69))

Abstract

Retroviral vectors are optimal gene transfer vehicles for gene therapy, since they integrate into the target cell genome and therefore can provide permanent expression of a therapeutic gene. This is a particularly desired feature in gene therapy of monogenic inherited diseases, in which the ultimate goal is a single gene transfer event that would lead to life-long correction of the genetic defect. Moreover, retroviral vectors are thoroughly characterized, and their extensive use in preclinical and clinical studies has verified their efficacy and safety.

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Author information

Authors and Affiliations

  1. A.I. Virtanen Institute for Molecular Sciences, Kuopio, Finland

    Jarmo Wahlfors

  2. Clinical Gene Therapy Branch, National Genome Research Institute, Bethesda, MD

    Richard A. Morgan

Authors
  1. Jarmo Wahlfors
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  2. Richard A. Morgan
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Editor information

Editors and Affiliations

  1. Brown University, Providence, RI

    Jeffrey R. Morgan

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© 2002 Humana Press Inc.

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Wahlfors, J., Morgan, R.A. (2002). Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System. In: Morgan, J.R. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 69. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-141-8:173

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  • DOI: https://doi.org/10.1385/1-59259-141-8:173

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-723-6

  • Online ISBN: 978-1-59259-141-1

  • eBook Packages: Springer Protocols

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