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Kinesin Protocols

  • Isabelle Vernos

Part of the Methods in Molecular Biology™ book series (MIMB, volume 164)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sergei A. Kuznetsov, Vladimir I. Gelfand
    Pages 1-7
  3. Laura M. Ginkel, Linda Wordeman
    Pages 21-41
  4. Maryanne F. Stock, David D. Hackney
    Pages 43-48
  5. Kimberly W. Waligora, Sharyn A. Endow
    Pages 49-55
  6. David D. Hackney, Wei Jiang
    Pages 65-71
  7. Sylvia Jeney, Ernst-Ludwig Florin, J. K. Heinrich Hörber
    Pages 91-108
  8. Arshad Desai, Claire E. Walczak
    Pages 109-121
  9. Erik Nielsen, Fedor Severin, Anthony A. Hyman, Marino Zerial
    Pages 133-146
  10. Gerardo Morfini, Ming-Ying Tsai, Györgyi Szebenyi, Scott T. Brady
    Pages 147-162
  11. Robert L. Morris, Heather M. Brown, Brent D. Wright, David J. Sharp, William Sullivan, Jonathan M. Scholey
    Pages 163-172
  12. Vladimir I. Gelfand, Nathalie Le Bot, M. Carolina Tuma, Isabelle Vernos
    Pages 191-204
  13. Lisa C. Lindesmith, Janardan Kumar, Michael P. Sheetz
    Pages 205-212
  14. François Nédélec, Thomas Surrey
    Pages 213-222
  15. Manfred Thormählen, Jens Müller, Eckhard Mandelkow
    Pages 223-233
  16. Back Matter
    Pages 255-258

About this book

Introduction

It is now clear that kinesin-like-proteins (KLP), generally thought to be responsible for the transport of cellular cargoes, are involved in many different cellular processes now being widely investigated. In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. Among the approaches discussed are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. Detailed examples of how to address functional studies are also presented, along with some very new methods for studying the role of KLP in the organization of microtubules in three dimensions. There are also advanced methods for the study of kinesins at the structural level.
Comprehensive and highly practical, Kinesin Protocols makes available all the key basic and cutting-edge methods needed successfully to study the multifaceted world of kinesin-like proteins and to explore their many functions.

Editors and affiliations

  • Isabelle Vernos
    • 1
  1. 1.European Molecular Biology LaboratoryHeidelbergGermany

Bibliographic information

  • DOI https://doi.org/10.1385/1592590691
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-766-3
  • Online ISBN 978-1-59259-069-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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