Skip to main content

Identification of Kinesin-Associated Proteins

  • Protocol
Kinesin Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 164))

Abstract

Kinesin can be found in both the soluble and vesicular fractions of cytosol. In both fractions, kinesin exists as a complex with other proteins that are hypothesized to provide regulation of the vesicle-binding and the ATPase activities of the kinesin heavy chain, effectively regulating kinesin-mediated vesicle motility. Thus, the identification of these kinesin-associated proteins would greatly increase our understanding of how kinesin function is regulated in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Gindhart, J. G., Jr. and Goldstein, L. S. (1998) Kinesin light chains are essential for axonal transport in Drosophila. J. Cell. Biol. 141, 443–454.

    Article  PubMed  CAS  Google Scholar 

  2. Cyr, J. L., Pfister, K. K., Bloom, G. S., Slaughter, C. A., and Brady, S. T. (1991) Molecular genetics of kinesin light chains: generation of isoforms by alternative splicing. Proc. Natl. Acad. Sci. USA 88, 10,114–10,118.

    Article  PubMed  CAS  Google Scholar 

  3. Khodjakov, A., Lizunova, E. M., Minin, A. A., Koonce, M. P., and Gyoeva, F. K. (1998) A specific light chain of kinesin associates with mitochondria in cultured cells. Mol. Biol. Cell 9, 333–343.

    PubMed  CAS  Google Scholar 

  4. Lizunova, E. M., Khodiakov, A. L., Gioeva, F. K., and Minin, A. A. (1998) One of the kinesin light chains is selectively associated with mitochondria. Mol. Biol. (Mosk.), 32, 154–161.

    CAS  Google Scholar 

  5. Rahman, A., Friedman, D. S., and Goldstein, L. S. (1998) Two kinesin light chain genes in mice. Identification and characterization of the encoded proteins. J. Biol. Chem. 273, 153,95–15,403; erratum: J. Biol. Chem. 273, 24,280.

    Article  PubMed  CAS  Google Scholar 

  6. Stenoien, D. L. and Brady, S. T. (1997) Immunochemical analysis of kinesin light chain function. Mol. Biol. Cell 8, 675–689.

    PubMed  CAS  Google Scholar 

  7. Verhey, K. J., Lizotte, D. L., Abramson, T., Barenboim, L., Schnapp, B. J., and Rapoport, T. A. (1998) Light chain-dependent regulation of Kinesin’s interaction with microtubules. J. Cell Biol. 143, 1053–1066.

    Article  PubMed  CAS  Google Scholar 

  8. Ingold, A., Cohn, S., and Scholey, J. (1988) Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657–2667.

    Article  PubMed  CAS  Google Scholar 

  9. Toyoshima, I., Yu, H., Steuer, E. R., and Sheetz, M. P. (1992) Kinectin, a major kinesin-binding protein on ER. J. Cell Biol. 118, 1121–1131.

    Article  PubMed  CAS  Google Scholar 

  10. Kumar, J., Erickson, H. P., and Sheetz, M. P. (1998) Ultrastructural and biochemical properties of the 120-kDa form of chick kinectin. J. Biol. Chem. 273, 31,738–31,743.

    Article  PubMed  CAS  Google Scholar 

  11. Kumar, J., Toyoshima, I., and Sheetz, M. P. (1998) Isolation and characterization of kinectin. Methods Enzymol. 298, 185–197.

    Article  PubMed  CAS  Google Scholar 

  12. Lindesmith, L., McIlvain, J. M., Jr., Argon, Y., and Sheetz, M. P. (1997) Phosphotransferases associated with the regulation of kinesin motor activity. J. Biol. Chem. 272, 22,929–22,933.

    Article  PubMed  CAS  Google Scholar 

  13. McIlvain, J. M., Jr., Burkhardt, J. K., Hamm-Alvarez S., Argon, Y., and Sheetz, M. P. (1994) Regulation of kinesin activity by phosphorylation of kinesin-associated proteins. J. Biol. Chem. 269, 19,176–19,182.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Humana Press Inc.

About this protocol

Cite this protocol

Lindesmith, L.C., Kumar, J., Sheetz, M.P. (2001). Identification of Kinesin-Associated Proteins. In: Vernos, I. (eds) Kinesin Protocols. Methods in Molecular Biology™, vol 164. Humana Press. https://doi.org/10.1385/1-59259-069-1:205

Download citation

  • DOI: https://doi.org/10.1385/1-59259-069-1:205

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-766-3

  • Online ISBN: 978-1-59259-069-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics