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RT-PCR for the Identification of Developmentally Regulated Novel Members of the Kinesin-like Superfamily

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Kinesin Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 164))

Abstract

The kinesin-like motor protein superfamily comprises to date about 100 members present in essentially all the major eukaryotic phyla (reviewed in ref. 1), including over 30 in the mouse alone (2). The common feature of the kinesin-like proteins is a highly conserved 350-amino-acid “motor domain” (30–40% sequence identity across species) that includes even more highly conserved ATP- and microtubule-binding sites (3).

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© 2001 Humana Press Inc.

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Santama, N. (2001). RT-PCR for the Identification of Developmentally Regulated Novel Members of the Kinesin-like Superfamily. In: Vernos, I. (eds) Kinesin Protocols. Methods in Molecular Biology™, vol 164. Humana Press. https://doi.org/10.1385/1-59259-069-1:9

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  • DOI: https://doi.org/10.1385/1-59259-069-1:9

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-766-3

  • Online ISBN: 978-1-59259-069-8

  • eBook Packages: Springer Protocols

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