Abstract
The kinesin-like motor protein superfamily comprises to date about 100 members present in essentially all the major eukaryotic phyla (reviewed in ref. 1), including over 30 in the mouse alone (2). The common feature of the kinesin-like proteins is a highly conserved 350-amino-acid “motor domain” (30–40% sequence identity across species) that includes even more highly conserved ATP- and microtubule-binding sites (3).
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References
Moore, J. D. and Endow, S. A. (1996) Kinesin proteins: a phylum of motors for microtubule-based motility. BioEssays 18, 207–219.
Hirokawa, N. (1998) Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science 279, 519–526.
Vale, R. D. (1991) Motor proteins, in Guidebook to the Cytoskeletal and Motor Proteins (Kreis, T. and Vale, R., eds.), Oxford University Press, Oxford, pp. 175–183.
Stewart, R. J., Pesavento, P. A., Woerpel, D. N., and Goldstein, L. S. (1991) Identification and partial characterization of six members of the kinesin superfamily in Drosophila. Proc. Natl. Acad. Sci. USA 88, 8470–8474.
Aizawa, H., Sekine, Y., Takemura, R., Zhang, Z., Nangaku, M., and Hirokawa, N. (1992) Kinesin family in murine central nervous system. J. Cell Biol. 119, 1287–1296.
Vernos I., Heasman J., and Wylie, C. (1993) Multiple kinesin-like transcripts in Xenopus oocytes. Dev. Biol. 157: 232–239.
Nakagawa, T., Tanaka, Y., Matsuoka, E., Kondo, S., Okada, Y., Noda, Y., et al. (1997) Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome. Proc. Natl. Acad. Sci. USA 94, 9654–9659.
Dotti, C. G., Sullivan, C. A. and Banker, G. A. (1988) The establishment of polarity by hippocampal neurons in culture. J. Neurosci. 8, 1454–1468.
Santama, N., Krijne-Locker, J., Griffiths, G., Noda, Y., Hirokawa, N. and Dotti C. G. (1998) KIF2β, a new kinesin superfamily protein, is associated with lysosomes and may be implicated in their centrifugal translocation. EMBO J. 17, 5855–5867.
Santama, N. (1997) In situ hybridisation for the detection of neuropeptide transmitters, in Methods in Molecular Biology, vol. 72: Neurotransmitter and Neuroreceptor Methods (Rayne, R. C., ed.), Humana Press, Totowa, NJ, pp. 159–170.
Saito, N., Okada, Y., Noda, Y., Kinoshita, Y., Kondo, S., and Hirokawa, N. (1997) KIFC2 is a novel neuron-specific C-terminal type kinesin superfamily motor for dendritic transport of multivesicular body-like organelles. Neuron 18, 425–438.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
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Santama, N. (2001). RT-PCR for the Identification of Developmentally Regulated Novel Members of the Kinesin-like Superfamily. In: Vernos, I. (eds) Kinesin Protocols. Methods in Molecular Biology™, vol 164. Humana Press. https://doi.org/10.1385/1-59259-069-1:9
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DOI: https://doi.org/10.1385/1-59259-069-1:9
Publisher Name: Humana Press
Print ISBN: 978-0-89603-766-3
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