Overview
- Editors:
-
-
Gerd P. Pfeifer
-
Department of Biology, Beckman Research Institute of the City of Hope, Duarte, USA
Access this book
Other ways to access
Table of contents (31 chapters)
-
Technologies for Detection of Mutations
-
- Damjan Glavač, Michael Dean
Pages 241-251
-
- Riccardo Fodde, Monique Losekoot
Pages 253-265
-
-
-
- Daizong Li, Nathalie van Orsouw, Chris Huang, Jan Vijg
Pages 291-305
-
- R. Bruce Wallace, Ching-I P. Lin, Antonio A. Reyes, Jimmie D. Lowery, Luis Ugozzoli
Pages 307-322
-
- Johan T. Den Dunnen, Pauline A. M. Roest, Rob B. Van Der Luijt, Frans B. L. Hogervorst
Pages 323-341
-
- Piroska E. Szabó, Gerd P. Pfeifer, Jeffrey R. Mann, Judith Singer-Sam
Pages 343-349
-
- Piroska E. Szabó, Jeffrey R. Mann, Gerald Forrest
Pages 351-370
-
Mammalian Systems for Mutation Analysis
-
Front Matter
Pages 371-371
-
-
- Veronica M. Maher, J. Justin McCormick
Pages 381-390
-
- Jan Vijg, George R. Douglas
Pages 391-410
-
- Johan G. de Boer, Heather L. Erfle, David Walsh, James Holcroft, Barry W. Glickman
Pages 411-429
-
- Fernando Aguilar, Peter Cerutti
Pages 431-438
-
Back Matter
Pages 439-441
About this book
Man-made carcinogens, natural genotoxic agents in the environment, as well as ionizing and ultraviolet radiation can damage DNA and are a constant threat to genome integrity. Throughout the evolution oflife, complex DNA repair systems have developed in all living organisms to cope with this damage. Unrepaired DNA lesions can promote genetic alterations (mutations) that may be linked to an altered phenotype, and, if growth-controlling genes are involved, these mutations can lead to cell transformation and the development of malignant tumors. Proto oncogenes and tumor suppressor genes may be critical targets for DNA damaging agents. In a number of animal model systems, correlations between exposure to a carcinogen, tumor develop ment, and genetic changes in tumor DNA have been established. To understand mutagenesis processes in more detail at the molecular level, we need to know the type and frequency of DNA adducts within cells, their distribution along genes and specific DNA sequences, as well as the rates at which they are repaired. We also need to know what types of mutations are produced and which gene positions are most prone to mutagenesis. This book provides a collection of techniques that are useful in mutagenesis research. The book is divided into three parts. In Part I, methods for DNA damage and repair analysis are provided.
Editors and Affiliations
-
Department of Biology, Beckman Research Institute of the City of Hope, Duarte, USA
Gerd P. Pfeifer