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Heteroduplex Analysis

  • Chapter
Technologies for Detection of DNA Damage and Mutations

Abstract

Many of the current methods used for mutation detection in genomic DNA rely on conformational changes in either double- or single-stranded DNA structure. Conformations can be distinguished by electrophoresis due to their different response to an electrical field. The rate of migration or mobility of DNA molecules through the electrical field depends not only on the shape of molecules, but also on the ionic strength, viscosity, and temperature of the medium in which the molecules are moving. For example, the single-stranded conformation polymorphism (SSCP) technique (Orita et al., 1989) depends on the altered mobility of single-stranded molecules and is a powerful diagnostic tool for detecting sequence variations.

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References

  • Artlich, A., Horn, M., Lorenz, B., Bhattacharyya, S., and Gal, S. (1992). Recurrent 3-bp deletion at codon 255/256 of the rhodopsin gene in a German pedigree with autosomal dominant retinitis pigmentosa. Am. J. Hum. Genet. 50:876–878.

    PubMed  CAS  Google Scholar 

  • Bhattacharyya, A., and Lilley, D. M. J. (1989). The contrasting structures of mismatched DNA sequences containing looped-out (bulges) and multiple mismatches (bubbles). Nucleic Acids Res. 17:6821–6840.

    Article  PubMed  CAS  Google Scholar 

  • Bidwell, J. L., Clay, T. M., Wood, N. A. P., Pursall, M. P., Martin, A. K., Bradley, B. A., and Hui, K. M. (1993). Rapid HLA-DR-Dw and DP matching by PCR fingerprinting and related DNA heteroduplex technologies, in:Handbook of HLA Typing Techniques (K. M. Hui and J. L. Bidwell, eds.), CRC Press, Boca Raton, pp. 99–116.

    Google Scholar 

  • Blum, H., Beier, H., and Gross, H. J. (1987). Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8:93–99.

    Article  CAS  Google Scholar 

  • Bourn, D., Carter, S. A., Mason, S., Evans, D. G. R., and Stachan, T. (1994). Germline mutations in the neurofi-bromatosis type 2 tumour suppressor gene. Hum. Mol. Genet. 3:813–816.

    Article  PubMed  CAS  Google Scholar 

  • Carrington, M., White, M. B., Dean, M., Mann, D., and Ward, F. E. (1992). The use of DNA heteroduplex patterns to map recombination within HLA class II region. Hum. Immunol. 33:114–121.

    Article  PubMed  CAS  Google Scholar 

  • Chevalier-Porst, F., Mathieu, M., and Bozon, D. (1993). Identification of three rare frameshift mutations in exon 13 of the cystic fibrosis gene:1918delGC, 2118del4, and 2372del8. Hum. Mol. Genet. 2:1071–1072.

    Article  PubMed  CAS  Google Scholar 

  • Claustres, M., Laussel, M., Desgeorges, M., Giansily, M., Culard, J.-F, Razakatsara, G., and Démaille, J. (1993). Analysis of the 27 exons and flanking regions of the cystic fibrosis gene:40 different mutations account for 91.2% of the mutant alleles in Southern France. Hum. Mol. Genet. 2:1209–1213.

    Article  PubMed  CAS  Google Scholar 

  • Cotton, R. G. H., Rodrigues, N. R., and Campbell, D. R. (1988). Reactivity of cytosine and thymine in single base pair mismatches with hydroxylamine and osmium tetroxide and its application to the study of mutations. Proc. Natl. Acad. Sci. USA 85:4397–4401.

    Article  PubMed  CAS  Google Scholar 

  • Cuppens, H., Loumi, O., Marynen, P., and Cassiman, J.-J. (1992). Identification of a new frameshift mutation and a duplication polymorphism in the CFTR gene in the Algerian population. Hum. Mol. Genet. 1:283–284.

    Article  PubMed  CAS  Google Scholar 

  • Dean, M. (1995). Resolving DNA mutations. Nature Genet. 9:103–104.

    Article  PubMed  CAS  Google Scholar 

  • Dodson, L. A., and Kant, J. A. (1991). Two temperature PCR and heteroduplex detection: Application to rapid cystic fibrosis screening. Mol. Cell. Probes 5:21–25.

    Article  PubMed  CAS  Google Scholar 

  • Friedl, W, Mandl, M., and Sengteller, M. (1993). Single-step screening method for the most common mutations in familial adenomatous polyposis. Hum. Mol. Genet. 2:1481–1482.

    Article  PubMed  CAS  Google Scholar 

  • Fukai, K., Holmes, S. A., Lucchese, N. J., Siu, V. M., Weleber, R. G., Schnur, R. E., and Spritz, R. A. (1994). Autosomal recessive ocular albinism associated with a functionally significant tyrosinase gene polymorphism. Nature Genet. 9:92–95.

    Article  Google Scholar 

  • Ganguly, A., and Prockop, D. J. (1990). Detection of single-base mutations by reaction of DNA heteroduplexes with a water-soluble carbodiimide followed by primer extension: Application to products from the polymerase chain reaction. Nucleic Acids Res. 18:3933–3939.

    Article  PubMed  CAS  Google Scholar 

  • Ganguly, A., Rock, M. J., and Prockop, D. J. (1993). Conformation-sensitive gel electrophoresis for rapid detection of single-base differences in doublestranded PCR products and DNA fragments: Evidence for solvent-induced bends in DNA heteroduplexes. Proc. Natl Acad. Sci. USA 90:10325–10329.

    Article  PubMed  CAS  Google Scholar 

  • Gayther, S. A., Wells, D., SenGupta, S. B., Champan, P., Neale, K., Tsioupra, K., and Delhanty, J. D. A. (1994). Regionally clustered APC mutations are associated with a severe phenotype and occur at a high frequency in new mutation cases of adenomatous polyposis coli. Hum. Mol. Genet. 3:53–56.

    Article  PubMed  CAS  Google Scholar 

  • Glavac, D., and Dean, M. (1993). Optimization of the single strand-conformation polymorphism (SSCP) technique for detection of point mutations. Hum. Mutat. 2:404–414.

    Article  PubMed  CAS  Google Scholar 

  • Guldberg, P., and Güttier, F. (1993). A simple method for identification of point mutations using denaturing gradient gel electrophoresis. Nucleic Acids Res. 9:2261–2262.

    Article  Google Scholar 

  • Hamzehloei, T., West, S. P., Chapman, P. D., Burn, J., and Curtis, A. (1994). Four novel germ-line mutations in the APC gene detected by heteroduplex analysis. Hum. Mol. Genet. 3:1023–1024.

    Article  PubMed  CAS  Google Scholar 

  • Highsmith, W. E. (1993). Carrier screening for cystic fibrosis. Clin. Chem. 39:706–707.

    Google Scholar 

  • Hule, M. L., Chen, A. S., Brooks, S. S., Grix, A., and Hirschhorn, R. (1994). A de novo 13 nt deletion, a newly identified C647W missense mutation and a deletion of exon 18 in infantile onset glycogen storage disease type II (GSDII). Hum. Mol Genet. 3:1081–1087.

    Article  Google Scholar 

  • Inglehearn, C. R., Keen, T. J., Bashir, R., Jay, M., Fitzke, M., Bird, A. C., Crombie, A., and Bhattacharyya, S. (1992). A completed screen for mutations of the rhodopsin gene in a panel of patients with autosomal dominant retinitis pigmentosa. Hum. Mol. Genet. 1:41–45.

    Article  PubMed  CAS  Google Scholar 

  • Joshua-Tor, L., Frolow, F., Appella, E., Hope, H., Rabinovich, D., and Sussman, J. L. (1992). Three-dimensional structures of bulge-containing DNA fragments. J. Mol. Biol. 225:397–431.

    Article  PubMed  CAS  Google Scholar 

  • Keen, J., Lester, D., Inglehearn, C., Curtis, A., and Bhattacharyya, S. (1991). Rapid detection of single base mismatches as heteroduplexes on Hydrolink gels. Trends Genet. 7:5.

    Article  PubMed  CAS  Google Scholar 

  • Kerem, B. S., Rommens, J. M., Buchanan, J. A., Markiewicz, D., Cox, T. K., Chakravarti, A., Buchwald, M., and Tsui, L.-C. (1989). Identification of the cystic fibrosis gene: Genetic analysis. Science 245:1073–1080.

    Article  PubMed  CAS  Google Scholar 

  • Legius, E., Hail, B. K., Wallace, M. R., Collins, F. S., and Glover, T. W. (1994). Ten base pair duplication in exon 38 of the NF1 gene. Hum. Mol. Genet. 3:829–830.

    Article  PubMed  CAS  Google Scholar 

  • Leoni, G. B., Rosatelli, M. C., Cossu, G., Pischedda, M. C., De Virgilliis, S., and Cao, A. (1992). A novel cystic fibrosis mutation: Deletion of seventeen nucleotides at the exon 10-intron 10 boundary of the CFTR gene, in a Sardinian patient. Hum. Mol. Genet. 1:83–84.

    Article  Google Scholar 

  • Lerman, L. S., and Silverstein, V. (1987). Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis. Methods Enzymol 155:482–501.

    Article  PubMed  CAS  Google Scholar 

  • McAllister, K. A., Grogg, K. M., Johnson, D. W., Gallione, C. J., Baldwin, M. A., Jackson, C. E., Helmbold, E. A., Markel, D. S., McKinnon, W. C., Murrell, J., McCormick, M. K., Pericak-Vance, M. A., Heutink, P., Oostra, B. A., Haitjema, T., Westerman, C. J. J., Porteous, M. E., Guttmacher, A. E., Letarte, M., and Marchuk, D. A. (1994). Endoglin, a TGF-beta binding protein of endothelial cells, is the gene for hereditary haemorrhagic telangiectasia type 1. Nature Genet. 8:345–351.

    Article  PubMed  CAS  Google Scholar 

  • Maekawa, M., Sudo, K., Kanno, T., Takayasu, S., Li, S. S.-L., Kitajima, M., and Matsuura, Y. (1994). A novel deletion mutation of lactate dehydrogenase A(M) gene in the fifth family with the enzyme deficiency. Hum. Mol. Genet. 3:825–826.

    Article  PubMed  CAS  Google Scholar 

  • Mandl, M., Paffenholz, R., Friedl, W., Caspari, R., Sengteller, M., and Propping, P. (1993). Frequency of common and novel inactivation APC mutations in 202 families with familial adenomatous polyposis. Hum. Mol. Genet. 3:825–826.

    Google Scholar 

  • Mandl, M., Kadmon, M., Sengteller, M., Caspari, R., Propping, P., and Fiedl, W. (1994). A somatic mutation in the adenomatous polyposis coli (APC) gene in peripheral blood cells—Implications for predictive diagnosis. Hum. Mol Genet. 3:1009–1011.

    Article  PubMed  CAS  Google Scholar 

  • Mary, J.-L. M., Bishop, T., Kolodner, R., Lipford, J. R., Kane, M., Weber, W, Torhorst, J., Mueler, H., Spycher, M., and Scott, R. J. (1994). Mutational analysis of the hMSH2 gene reveals a three base pair deletion in a family predisposed to colorectal cancer development. Hum. Mol Genet. 3:2067–2069.

    PubMed  CAS  Google Scholar 

  • Mashal, R. D., Koontz, J., and Sklar, J. (1995). Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases. Nature Genet. 9:177–183.

    Article  PubMed  CAS  Google Scholar 

  • Meins, M., Griming, G., Blankenagel, A., Krastel, H., Reck, B., Fuchs, S., Schwinger, E., and Gal, A. (1993). Heterozygous ‘null allele’ mutation in the human peripherin/RDS gene. Hum. Mol Genet. 2:2181–2182.

    Article  PubMed  CAS  Google Scholar 

  • Meitinger, T., Golla, A., Dörner, C., Deufel, A., Aulehla-Scholz, A., Boehm, I., Reinhardt, D., and Deufel, T.(1993). In frame deletion (deltaF311) within a short trinucleotide repeat of the first transmembrane region of the cystic fibrosis gene. Hum. Mol. Genet. 2:2173–2174.

    Article  PubMed  CAS  Google Scholar 

  • Molinari, R. J., Conners, M., and Shorr, R. G. L. (1993). Hydrolink gels for electrophoresis, in:Advances in Electrophoresis, Volume 6 (A. Chrambach, M. J. Dunn, and B. J. Radola, eds.), VCH Publishers, New York, pp. 44–60.

    Google Scholar 

  • Myers, R. M., Larin, Z., and Maniatis, T. (1985). Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science 230:1242–1249.

    Article  PubMed  CAS  Google Scholar 

  • Myers, R.M., Maniatis, T., and Lerman, L. (1987). Detection and localization of single base changes by denaturing gradient gel electrophoresis. Methods Enzymol 155:501–527.

    Article  PubMed  CAS  Google Scholar 

  • Nagamine, C. M., Chan, K., and Lau, Y.-F. C. (1989). A PCR artifact: Generation of heteroduplexes. Am. J. Hum. Genet. 45:337–339.

    PubMed  CAS  Google Scholar 

  • Olds, R. J., Lane, D. A., Beresford, C. J., Abilgaard, U., Hughes, P. M., and Thein, S. L. (1993). A recurrent deletion in the antithrombin gene, AT106–109 (–6 bp), identified by DNA heteroduplex detection. Genomics 16:298–299.

    Article  PubMed  CAS  Google Scholar 

  • Olivas, M. W., and Maher, L. J. (1994). Analysis of duplex DNA by triple helix formation: Application to detection of a p53 microdeletion. Biotechniques 16:128–132.

    PubMed  CAS  Google Scholar 

  • Orita, M., Suzuki, Y, Sekiya, T., and Hayashi, K. (1989). Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5:874–879.

    Article  PubMed  CAS  Google Scholar 

  • Ouahchi, K., Arita, M., Kayden, H., Hentati, K., Hamida, M. B., Sokol, R., Arai, H., Ionoue, K., Mandel, J.-L., and Koenig, M. (1995). Ataxia with isolated vitamin E deficiency is caused by mutations in the alpha-tocopherol transfer protein. Nature Genet. 9:141–145.

    Article  PubMed  CAS  Google Scholar 

  • Paffenholz, R., Mandl, M., Caspari, R., Sengteller, M., Propping, P., and Friedl, W. (1994). Eleven novel germline mutations in the adenomatous polyposis coli (APC) gene. Hum. Mol. Genet. 3:1703–1704.

    Article  PubMed  CAS  Google Scholar 

  • Paul, P., Letteboer, T., Gelbert, L., Groden, J., White, R., and Coppes, M. J. (1993). Identical APC exon 15 mutations result in a variable phenotype in familial adenomatous polyposis. Hum. Mol. Genet. 2:925–931.

    Article  PubMed  CAS  Google Scholar 

  • Perry, D. J., and Carrell, R. W. (1992). Hydrolink gels: A rapid and simple approach to the detection of DNA mutations in thromboembolic disease. J. Clin. Pathol. 45:158–160.

    Article  PubMed  CAS  Google Scholar 

  • Prior, T. W., Papp, A. C., Snyder, P. J., and Sedra, M. S. (1993a). Detection of an exon 53 polymorphism in the dystrophin gene. Hum. Genet. 92:302–304.

    Article  PubMed  CAS  Google Scholar 

  • Prior, T. W., Papp, A. C., Snyder, P. J., Burghes, A. H. M., Sedra, M. S., Western, L. M., Bartolo, C., and Mendell, J. R. (1993b). Exon 44 nonsense mutation in two Duchenne muscular dystrophy brothers detected by heteroduplex analysis. Hum. Mutat. 2:192–195.

    Article  PubMed  CAS  Google Scholar 

  • Prior, T. W, Bartolo, C., Papp, A. C., Snyder, P. J., Sedra, M. S., Burghes, A. H. M., and Mendell, J. R. (1994). Identification of a missense mutation, single base deletion and a polymorphism in the dystrophin exon 16. Hum. Mol. Genet. 3:1173–1174.

    Article  PubMed  CAS  Google Scholar 

  • Ravnik-Glavaö, M., Glavaé, D., and Dean, M. (1994). Sensitivity of single-strand conformation polymorphism (SSCP) and heteroduplex method (HA) for mutation detection in the cystic fibrosis gene. Hum. Mol. Genet. 3:801–807.

    Article  Google Scholar 

  • Rommens, J. M., Kerem, B.-S., Greer, W, Chang, P., Tsui, L.-C., and Ray, P. (1990). Rapid nonradioactive detection of the major cystic fibrosis mutation. Am. J. Hum. Genet. 46:395–396.

    PubMed  CAS  Google Scholar 

  • Saad, F. A., Vitiello, L., Merlini, L., Mostacciuolo, M. L., Olivero, S., and Danieli, G. A. (1992). A 3’ consensus splice mutation in the human dystrophin gene detected by a screening for intra-exonic deletions. Hum. Mol. Genet. 1:345–346.

    Article  PubMed  CAS  Google Scholar 

  • Sainz, J., Huynh, D. P., Figuerosa, K., Ragge, N. K., Baser, M. E., and Pulst, S. M. (1994). Mutations of the neurofibromatosis type 2 gene and lack of the gene product in vestibular schwannomas. Hum. Mol. Genet. 3:885–891.

    Article  PubMed  CAS  Google Scholar 

  • Sheffield, V. C., Cox, D. R., Lerman, L. S., and Myers, R. M. (1989). Attachment of a 40-base pair G+C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc. Natl. Acad. Sci. USA 86:232–236.

    Article  PubMed  CAS  Google Scholar 

  • Shen, M. H., Harper, P. S., and Upadhyaya, M. (1992). Neurofibromatosis type 1 (NF1):The search for mutations by PCR-heteroduplex analysis on Hydrolink gels. Hum. Mol. Genet. 1:735–740.

    Article  PubMed  Google Scholar 

  • Sorrentino, R., Iannicola, C., Costanzi, S., Chersi, A., and Roberto, T. (1991). Detection of complex alleles by direct analysis of DNA heteroduplexes. Immunogenetics 33:118–123.

    Article  PubMed  CAS  Google Scholar 

  • Sorrentino, R., Cascino, I., and Tosi, R. (1992a). Subgrouping of DR4 alleles by DNA heteroduplex analysis. Hum. Immunol. 33:18–23.

    Article  PubMed  CAS  Google Scholar 

  • Sorrentino, R., Potolicchio, I., Ferrara, G. B., and Tosi, R. (1992b). A new approach to HLA-DPB1 typing combining DNA heteroduplex analysis with allele-specific amplification and enzyme restriction. Immunogenetics 36:248–254.

    Article  PubMed  CAS  Google Scholar 

  • Tassabehji, M., Read, A. P., Newton, V. E., Harris, R., Balling, R., Gruss, P., and Strachan, T. (1992). Waardenburg’s syndrome patients have mutations in the human homologue. Nature 355:635–636.

    Article  PubMed  CAS  Google Scholar 

  • Tassabehji, M., Newton, V. E., and Read, A. P. (1994). Waardenburg’s syndrome type 2 caused by mutations in the human microphthalmia (MITF) gene. Nature Genet. 8:251–255.

    Article  PubMed  CAS  Google Scholar 

  • Triggs-Raine, B. L., and Gravel, R. A. (1990). Diagnostic heteroduplexes: Simple detection of carriers of a 4-bp insertion mutation in Tay-Sachs disease. Am. J. Hum. Genet. 46:183–184.

    PubMed  CAS  Google Scholar 

  • Tsukamoto, H., Inui, K., Matsuoka, T., Yanagihara, L, Fukushima, H., and Okada, S. (1994). One base deletion in the cysteine-rich domain of the dystrophin gene in Duchenne muscular dystrophy patients. Hum. Mol. Genet. 3:995–996.

    Article  PubMed  CAS  Google Scholar 

  • Upadhyaya, M., Shen, M., Cherryson, Farnham, J., Maynard, J., Huson, S. M., and Harper, P. S. (1992). Analysis of mutations at the neurofibromatosis 1 (NF1) locus. Hum. Mol. Genet. 1:735–740.

    Article  PubMed  CAS  Google Scholar 

  • van den Akker, E., Braun, J. E. F, Pals, G., Lafleur, M. V. M., and Retel, J. (1992). Single base mutations can be unequivocally and rapidly detected by analysis of DNA heteroduplexes, obtained with deletion-mutant instead of wild-type DNA. Nucleic Acids Res. 24:6745–6746.

    Article  Google Scholar 

  • Wang, Y.-H., Barker, P., and Griffith, J. J. (1992). Visualization of diagnostic heteroduplex DNAs from cystic fibrosis deletion hétérozygotes provides an estimate of the kinking of DNA by bulged bases. J. Biol. Chem. 267:4911–4915.

    PubMed  CAS  Google Scholar 

  • White, M. B., Amos, J., Hsu, J. M., Gerrard, B., Finn, P., and Dean, M. (1990). A frameshift mutation in the cystic fibrosis gene. Nature 344:665–667.

    Article  PubMed  CAS  Google Scholar 

  • White, M. B., Carvalho, M., Derse, D., O’Brien, S. J., and Dean, M. (1992). Detecting single base substitutions as heteroduplex polymorphisms. Genomics 12:301–306.

    Article  PubMed  CAS  Google Scholar 

  • Williams, C. J., Rock, M., Considine, E., McCarron, S., Gow, P., Ladda, R., McLain, D., Michels, V. M., Murphy, W., Prockop, D. J., and Ganguly, A. (1995). Three new point mutations in type II procollagen (COL2A1) and identification of a fourth family with the COL2A1 Arg519→Cys base substitution using conformation sensitive gel electrophoresis. Hum. Mol. Genet. 4:309–312.

    Article  PubMed  CAS  Google Scholar 

  • Winterpacht, A., Schwarze, U., Mundlos, S., Menger, H., Spranger, J., and Zabei, B. (1994). Alternative splicing as the result of a type II procollagen gene (COL2A1) mutation in a patient with Kniest dysplasia. Hum. Mol. Genet. 3:1891–1893.

    Article  PubMed  CAS  Google Scholar 

  • Wood, N., Tyfield, L., and Bidwell, J. (1993a). Rapid classification of phenylketonuria genotypes by analysis of heteroduplexes generated by PCR-amplifiable synthetic DNA. Hum. Mutat. 2:131–137.

    Article  PubMed  CAS  Google Scholar 

  • Wood, N., Standen, G., Hows, J., Bradley, B., and Bidwell, J. (1993b). Diagnosis of sickle-cell disease with a universal heteroduplex generator. Lancet 342:1519–1520.

    Article  PubMed  CAS  Google Scholar 

  • Wood, N., Standen, G., Old, J., and Bidwell, J. (1995). Genotyping for haemoglobins S and C by DNA crossmatching with a universal heteroduplex generator. Hum. Mutat. 5:166–172.

    Article  PubMed  CAS  Google Scholar 

  • Woodson, S. A., and Crothers, D. M. (1988). Structural model for an oligonucleotide containing a bulged guanosine by NMR and energy minimization. Biochemistry 27:3130–3141.

    Article  PubMed  CAS  Google Scholar 

  • Youil, R., Kemper, B., and Cotton, R. G. H. (1995). Screening for mutations by enzyme mismatch cleavage using T4 endonuclease VII. Proc. Natl. Acad. Sci. USA 92:87–91.

    Article  PubMed  CAS  Google Scholar 

  • Zimmerman, P. A., Carrington, M. N., and Nutman, T. B. (1993). Exploiting structural differences among heteroduplex molecules to simplify genotyping the DQA1 and DQB1 alleles in human lymphocyte typing. Nucleic Acids Res. 21:4541–4547.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Medical Faculty, Institute of Pathology, Ljubljana, Slovenia

    Damjan Glavač

  2. Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland, 21702, USA

    Michael Dean

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  1. Damjan Glavač
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  1. Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California, 91010, USA

    Gerd P. Pfeifer

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© 1996 Springer Science+Business Media New York

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Glavač, D., Dean, M. (1996). Heteroduplex Analysis. In: Pfeifer, G.P. (eds) Technologies for Detection of DNA Damage and Mutations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0301-3_18

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  • DOI: https://doi.org/10.1007/978-1-4899-0301-3_18

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