Single-Strand Conformation Polymorphism Analysis
Nucleotide sequences of genomic DNA in cells can be altered by a variety of factors. If the alterations do not influence the growth and development of cells, the nucleotide sequences present in germinal cells can be transferred to progenies. If the nucleotide sequence changes influence normal cellular functions, they result in diverse diseases, such as cancers and hereditary diseases in humans.
KeywordsPolymerase Chain Reaction Mobility Shift Polymerase Chain Reaction Buffer Polymerase Chain Reaction Solution Acrylamide Solution
Unable to display preview. Download preview PDF.
- Dockhorn-Dworniczak, B., Dworniczak, B., Brömmelkamp, L., Bülles, J., Horst, J., and Böcker, W. W. (1991). Non-isotopic detection of single strand conformation polymorphism (PCR-SSCP); a rapid and sensitive technique in diagnosis of phenylketonuria. Nucleic Acids Res. 19:2500.PubMedCrossRefGoogle Scholar
- Hata, A., Robertson, M., Emi, M., and Lalouel, J.-M. (1990). Direct detection and automated sequencing of individual alleles after electrophoretic strand separation: Identification of a common nonsense mutation in exon 9 of the human lipoprotein lipase gene. Nucleic Acids Res. 18:5407–5411.PubMedCrossRefGoogle Scholar
- Mashiyama, S., Sekiya, T., and Hayashi, K. (1990). Screening of multiple DNA samples for detection of sequence changes. Technique 2:304–306.Google Scholar
- Sheffield, V. C., Cox, D. R., Lerman, D. R., and Myers, R. M. (1989). Attachment of a 40-base-pair G+C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes. Proc. Natl. Acad. Sci. USA 86:232–236.PubMedCrossRefGoogle Scholar