Genotypic Mutation Assay (RFLP/PCR)

  • Fernando Aguilar
  • Peter Cerutti


Mutational spectrum analysis of DNA has gained considerable interest among molecular toxicologists as an attempt to correlate DNA alterations caused by endogenous and exogenous mutagens with epidemiological studies of cancer risk factors (Shields and Harris, 1991). Mutational abnormalities in cancer-related genes may suggest mechanisms of carcinogenesis in specific organs or tissues. Furthermore, mutations in proto-oncogenes and tumor suppressor genes in premalignant tissues may become a useful parameter for risk assessment (Greenblatt et al., 1994; Harris, 1993).


Authentic Mutant Genotypic Mutation Base Pair Change Mutant Standard Incubation Oven 
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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Fernando Aguilar
    • 1
  • Peter Cerutti
    • 1
  1. 1.Nestec SALausanne 26Switzerland

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