Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1531)
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Table of contents (19 protocols)
Keywords
About this book
This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.
Editors and Affiliations
Bibliographic Information
Book Title: Type 3 Secretion Systems
Book Subtitle: Methods and Protocols
Editors: Matthew L. Nilles, Danielle L. Jessen Condry
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6649-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2017
Hardcover ISBN: 978-1-4939-6647-9Published: 12 November 2016
Softcover ISBN: 978-1-4939-8260-8Published: 27 June 2018
eBook ISBN: 978-1-4939-6649-3Published: 11 November 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 224
Number of Illustrations: 9 b/w illustrations, 23 illustrations in colour
Topics: Microbiology
Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma