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Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins

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Type 3 Secretion Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1531))

Abstract

The ability to express and purify recombinant needle proteins from the Type III Secretion System (T3SS) of many gram-negative bacteria has allowed us to develop novel experimental approaches, both in vitro and in vivo, to identify unique roles for T3SS in bacterial pathogenesis. In addition, these purified needle proteins have shown to be promising immunotherapies acting as both protective antigens and adjuvants, presumably due to their immune activating properties. Here, we describe the expression and purification of recombinant T3SS needle proteins.

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Author information

Authors and Affiliations

  1. Department of Biomedical Sciences, University of North Dakota, 1301 North Columbia Road, Stop 9037, Grand Forks, ND, 58203, USA

    Travis D. Alvine

  2. Department of Microbiology, University of Chicago, Chicago, IL, USA

    Patrick Osei-Owusu

  3. Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA

    Danielle L. Jessen Condry & Matthew L. Nilles

Authors
  1. Travis D. Alvine
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  2. Patrick Osei-Owusu
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  3. Danielle L. Jessen Condry
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  4. Matthew L. Nilles
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Corresponding author

Correspondence to Matthew L. Nilles .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, School of Medicine and Health Sciences University of North Dakota, Grand Forks, North Dakota, USA

    Matthew L. Nilles

  2. Department of Biomedical Sciences, School of Medicine and Health Sciences University of North Dakota, Grand Forks, North Dakota, USA

    Danielle L. Jessen Condry

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© 2017 Springer Science+Business Media New York

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Alvine, T.D., Osei-Owusu, P., Condry, D.L.J., Nilles, M.L. (2017). Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins. In: Nilles, M., Condry, D. (eds) Type 3 Secretion Systems. Methods in Molecular Biology, vol 1531. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6649-3_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6649-3_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6647-9

  • Online ISBN: 978-1-4939-6649-3

  • eBook Packages: Springer Protocols

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