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Detection of Protein Interactions in T3S Systems Using Yeast Two-Hybrid Analysis

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Type 3 Secretion Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1531))

Abstract

Two-hybrid systems, sometimes termed interaction traps, are genetic systems designed to find and analyze interactions between proteins. The most common systems are yeast based (commonly Saccharomyces cerevisae) and rely on the functional reconstitution of the GAL4 transcriptional activator. Reporter genes, such as the lacZ gene of Escherichia coli (encodes β-galactosidase), are placed under GAL4-dependent transcriptional control to provide quick and reliable detection of protein interactions. In this method the use of a yeast-based two-hybrid system is described to study protein interactions between components of type III secretion systems.

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Authors and Affiliations

  1. Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA

    Matthew L. Nilles

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  1. Matthew L. Nilles
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Correspondence to Matthew L. Nilles .

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Editors and Affiliations

  1. Department of Biomedical Sciences, School of Medicine and Health Sciences University of North Dakota, Grand Forks, North Dakota, USA

    Matthew L. Nilles

  2. Department of Biomedical Sciences, School of Medicine and Health Sciences University of North Dakota, Grand Forks, North Dakota, USA

    Danielle L. Jessen Condry

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Nilles, M.L. (2017). Detection of Protein Interactions in T3S Systems Using Yeast Two-Hybrid Analysis. In: Nilles, M., Condry, D. (eds) Type 3 Secretion Systems. Methods in Molecular Biology, vol 1531. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6649-3_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6649-3_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6647-9

  • Online ISBN: 978-1-4939-6649-3

  • eBook Packages: Springer Protocols

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