Abstract
Measuring immune responses against type III secretion and related molecules has been made easier with the advent of reporter cell lines. For example, the THP-1-XBlue and HEK-Blue cells from InvivoGen provide easy detection of gene activation under NF-kB and AP-1 control. In addition, many of these cells have been engineered to express specific pattern recognition receptors (PRRs) helping to elucidate activation pathways. In addition, the THP-1 parent cell line from ATCC provides a useful tool for evaluating cytokine release upon stimulation. This chapter will outline using X-blue reporter cell lines, setting up experiments to measure cytokine secretion, and general guidelines for cytokine quantification.
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References
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THP-1 (TIB-202) Product Sheet, ATCC
THP-1-XBlue Technical Data Sheet, InvivoGen
HEK-Blue-hTLR4 Technical Data Sheet, InvivoGen
HEK-Blue-hTLR2 Technical Data Sheet, InvivoGen
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© 2017 Springer Science+Business Media New York
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Knopick, P.L., Bradley, D.S. (2017). Detecting Immune Responses to Type III Secretion Systems. In: Nilles, M., Condry, D. (eds) Type 3 Secretion Systems. Methods in Molecular Biology, vol 1531. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6649-3_14
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DOI: https://doi.org/10.1007/978-1-4939-6649-3_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6647-9
Online ISBN: 978-1-4939-6649-3
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