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Type 3 Secretion Systems pp 155–164Cite as

A Method for Characterizing the Type III Secretion System’s Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1531))

Abstract

The type III (T3S) secretion system of many gram-negative bacteria is a surface-exposed protein secretion apparatus used to directly inject bacterial effector molecules into eukaryotic cells. These effector molecules contribute to bacterial pathogenesis in many ways, and have been shown to be crucial for infectivity. Here, we describe a protocol for using homologous recombination to generate T3S system mutants to assess the role of different T3S system proteins in bacterial pathogenesis.

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Author information

Authors and Affiliations

  1. Department of Microbiology, University of Chicago, Chicago, IL, USA

    Patrick Osei-Owusu

  2. Department of Biomedical Sciences, University of North Dakota, 1301 North Columbia Road, Stop 9037, Grand Forks, ND, 58203, USA

    David S. Bradley & Travis D. Alvine

  3. Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA

    Matthew L. Nilles

Authors
  1. Patrick Osei-Owusu
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  2. Matthew L. Nilles
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  3. David S. Bradley
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  4. Travis D. Alvine
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Corresponding author

Correspondence to Matthew L. Nilles .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, School of Medicine and Health Sciences University of North Dakota, Grand Forks, North Dakota, USA

    Matthew L. Nilles

  2. Department of Biomedical Sciences, School of Medicine and Health Sciences University of North Dakota, Grand Forks, North Dakota, USA

    Danielle L. Jessen Condry

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Osei-Owusu, P., Nilles, M.L., Bradley, D.S., Alvine, T.D. (2017). A Method for Characterizing the Type III Secretion System’s Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants. In: Nilles, M., Condry, D. (eds) Type 3 Secretion Systems. Methods in Molecular Biology, vol 1531. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6649-3_13

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  • DOI: https://doi.org/10.1007/978-1-4939-6649-3_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6647-9

  • Online ISBN: 978-1-4939-6649-3

  • eBook Packages: Springer Protocols

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