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Cytochrome P450 Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Ian R. Phillips

    1. Department of Biochemistry, Queen Mary and Westfie1d College, University of London, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Elizabeth A. Shephard

    1. Department of Biochemistry and Molecular Biology, University College, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 107)

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Table of contents (44 protocols)

  1. An Isocratic High-Performance Liquid Chromatographic Assay for CYP7Al-Catalyzed Cholesterol 7α-Hyciroxylation

    • David J. Waxman, Thomas K. H. Chang
    Pages 169-174

  2. Use of 7-Ethoxycoumarinto Monitor Multiple Enzymesin the Human CYP1, CYP2, and CYP3 Families

    • David J. Waxman, Thomas K. H. Chang
    Pages 175-180

  3. Expression of Eukaryotic Cytochromes P450 in E. coli

    • Christopher M. Jenkins, Irina Pikuleva, Michael R. Waterman
    Pages 181-194

  4. Expression of Mammalian Cytochromes P450 in Yeast

    • Yoshiyasu Yabusaki
    Pages 195-202

  5. Expression of Cytochromes P450 in a Baculovirus System

    • Steven R. Hood, Girish Shah, Peter Jones
    Pages 203-218

  6. Expression of Cytochromes P450 in Mammalian Cells

    • Els de M. Groene
    Pages 219-226

  7. Monoclonal Antibodies to Cytochromes P450

    • Harry V. Gelboin, Magang Shou, Inna Goldfarb, Tian J. Yang, Kristopher Krausz
    Pages 227-238

  8. Targeting Antipeptide Antibodies Towards Cytochrome P450 Enzymes

    • Robert J. Edwards
    Pages 239-250

  9. Signals for Retention of Cytochrome P450 in the Endoplasmic Reticulum

    • Elzbieta Skorupa, Byron Kemper
    Pages 251-266

  10. Quantification of Cytochrome P450 Gene Expression by RNase Protection Analysis

    • Colin N. A. Palmer
    Pages 267-277

  11. Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing

    • Vera Rogiers, Antoine Vercruysse
    Pages 279-294

  12. Isolation of Rat Hepatocytes

    • Karen De Smet, Sonja Beken, Tamara Vanhaecke, Marleen Pauwels, Antoine Vercruysse, Vera Rogiers
    Pages 295-302

  13. Collagen-Gel Cultures of Rat Hepatocytes:

    • Sonja Beken, Tamara Vanhaecke, Karen De Smet, Marleen Pauwels, Antoine Vercruysse, Vera Rogiers
    Pages 303-310

  14. Rat Hepatocyte Cultures

    • Tamara Vanhaecke, Karen De Smet, Sonja Beken, Marleen Pauwels, Antoine Vercruysse, Vera Rogiers
    Pages 311-318

  15. Isolation of Periportal and Pericentral Hepatocytes

    • Rolf Gebhardt
    Pages 319-328

  16. Perifusion Culture of Hepatocytes

    • Rolf Gebhardt
    Pages 329-340

  17. Human Hepatocyte Culture

    • Jean Bernard Ferrini, Jean-Claude Ourlin, Lydiane Pichard, Gérard Fabre, Patrick Maurel
    Pages 341-352

  18. Reconstruction of Human Epidermis In Vitro

    • Marie-Cécile Lenoir-Viale
    Pages 353-360

  19. Gene Transfer and Expression

    • Frank Gaunitz, Monika Papke
    Pages 361-370

  20. Lipid-Mediated Gene Transfer into Normal Adult Human Hepatocytes in Primary Culture

    • Jean-Claude Ourlin, Marie José Vilarem, Martine Daujat, Marie Cécile Harricane, Patrick Maurel
    Pages 371-380
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About this book

In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphisms. In addition, because of the growing importance of in vitro systems in pharmacological toxicology, the book contains techniques for the culture of rodent and human hepatocytes and human epidermis. Cytochrome P450 induction as a biomarker for environmental pollution and the generation of mice with targeted gene disruptions complete this exhaustive collection of core techniques.

Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharmacology, environmental biology, biochemistry, and molecular biology-all the tools needed to elucidate the crucial biological role played by cytochromes P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants.



Editors and Affiliations

  • Department of Biochemistry, Queen Mary and Westfie1d College, University of London, UK

    Ian R. Phillips

  • Department of Biochemistry and Molecular Biology, University College, London, UK

    Elizabeth A. Shephard

Bibliographic Information

  • Book Title: Cytochrome P450 Protocols

  • Editors: Ian R. Phillips, Elizabeth A. Shephard

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896035190

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • eBook ISBN: 978-1-59259-580-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 482

  • Number of Illustrations: 49 b/w illustrations

  • Topics: Biochemistry, general

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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