Abstract
Collagen-gel cultures are relatively new in vitro models for long-term culture of functional hepatocytes. In these models, the cells are cultured between two layers of the extracellular matrix protein, hydrated collagen type I.
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References
Beken, S., Pauwels, M., Pahernik, S, Koebe, H.-G., Vercruysse, A. and Rogiers, V. (1997) Collagen gel sandwich and immobilization cultures of rat hepatocytes. problems encountered in expressing glutathione S-transferase activities Toxicol In Vitro 11 741–752.
Beken, S, Pahernik, S, Koebe, H-G., Vercruysse, A., and Rogiers, V (1997) Cell morphology, albumin secretion, and glutathione S-transferase expression in collagen gel sandwich and immobilization cultures of rat hepatocytes Toxicol In Vitro 11, 409–416.
Dunn, J C. Y, Tompkins, R. G., and Yarmush, M.L. (1991) Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol. Prog 7, 237–245.
Koebe, H.-G, Pahernik, S, Eyer, P., and Schildberg, F.-W (1994) Collagen gel immobilization a useful cell culture technique for long-term metabolic studies in human hepatocytes Xenobiotica 24, 95–107.
Eisdale, T. and Bard, J (1972) Collagen substrata for studies on cell behavior. J Cell Biol. 54, 626–637.
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© 1998 Humana Press Inc.
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Beken, S., Vanhaecke, T., De Smet, K., Pauwels, M., Vercruysse, A., Rogiers, V. (1998). Collagen-Gel Cultures of Rat Hepatocytes:. In: Phillips, I.R., Shephard, E.A. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology™, vol 107. Humana Press. https://doi.org/10.1385/0-89603-519-0:303
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DOI: https://doi.org/10.1385/0-89603-519-0:303
Publisher Name: Humana Press
Print ISBN: 978-0-89603-519-5
Online ISBN: 978-1-59259-580-8
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