Cytochrome P450 Protocols

  • Ian R. Phillips
  • Elizabeth A. Shephard

Part of the Methods in Molecular Biology™ book series (MIMB, volume 107)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. David R. Nelson
    Pages 15-24
  3. John B. Schenkman, Ingela Jansson
    Pages 25-34
  4. F. Peter Guengerich, Martha V. Martin
    Pages 35-54
  5. Margaret Warner, Jan-Åke Gustafsson
    Pages 69-76
  6. F. Peter Guengerich, Natalie A. Hosea, Martha V. Martin
    Pages 77-84
  7. Tsutomu Shimada, Hiroshi Yamazaki
    Pages 85-94
  8. Thomas K. H. Chang, David J. Waxman
    Pages 95-102
  9. David J. Waxman, Thomas K. H. Chang
    Pages 111-116
  10. Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 123-128
  11. Charles L. Crespi, Thomas K. H. Chang, David J. Waxman
    Pages 129-134
  12. Thomas K. H. Chang, Charles L. Crespi, David J. Waxman
    Pages 147-152

About this book


In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphisms. In addition, because of the growing importance of in vitro systems in pharmacological toxicology, the book contains techniques for the culture of rodent and human hepatocytes and human epidermis. Cytochrome P450 induction as a biomarker for environmental pollution and the generation of mice with targeted gene disruptions complete this exhaustive collection of core techniques.

Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharmacology, environmental biology, biochemistry, and molecular biology-all the tools needed to elucidate the crucial biological role played by cytochromes P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants.

Editors and affiliations

  • Ian R. Phillips
    • 1
  • Elizabeth A. Shephard
    • 2
  1. 1.Department of Biochemistry, Queen Mary and Westfie1d CollegeUniversity of LondonUK
  2. 2.Department of Biochemistry and Molecular BiologyUniversity CollegeLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 1998
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-519-5
  • Online ISBN 978-1-59259-580-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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