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Human Hepatocyte Culture

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Cytochrome P450 Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 107))

Abstract

Primary culture of hepatocytes is an in vitro model widely used to investigate various aspects of liver physiology and pathology (1). In particular, such cultures have been extensively used for assessing the expression and function of drug-metabolizing enzymes including cytochromes P450 (CUP), drug metabolism, drug-drug interactions, and the mechanisms of cytotoxtcity and genotoxicity. Most of these studies have been carried out with rodent hepatocytes. However, because of species-specificity in both the regulation and activity of drug-metabolizing enzymes, extrapolation from animals to humans is not generally possible. For this reason, several groups have developed human hepatocyte culture systems (2–8). The technique used to isolate human hepatocytes is based on the two-step collagenase perfusion first mtroduced by Berry and Friend (9) and modified by Seglen (10). Originally performed in situ for obtaining hepatocytes from the adult rat, this technique of perfusion has been adapted to the ex vivo treatment of human liver tissue. The aim of this chapter is to describe the authors′ experience in the isolation of hepatocytes from human liver tissue and the preparation of short- and long-term cultures

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Author information

Authors and Affiliations

  1. Department of Metabolism and Pharmacokinetics, Sanofi-Recherche, Montpellier, France

    Jean Bernard Ferrini, Lydiane Pichard & Gérard Fabre

  2. Biozentrum der Universitat Basel, ABT, Pharmakologie, Basel, Switzerland

    Jean-Claude Ourlin

  3. INSERM U, Montpellier, France

    Patrick Maurel

Authors
  1. Jean Bernard Ferrini
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  2. Jean-Claude Ourlin
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  3. Lydiane Pichard
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  4. Gérard Fabre
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  5. Patrick Maurel
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, Queen Mary and Westfie1d College, University of London, UK

    Ian R. Phillips

  2. Department of Biochemistry and Molecular Biology, University College, London, UK

    Elizabeth A. Shephard

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© 1998 Humana Press Inc.

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Ferrini, J.B., Ourlin, JC., Pichard, L., Fabre, G., Maurel, P. (1998). Human Hepatocyte Culture. In: Phillips, I.R., Shephard, E.A. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology™, vol 107. Humana Press. https://doi.org/10.1385/0-89603-519-0:341

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  • DOI: https://doi.org/10.1385/0-89603-519-0:341

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-519-5

  • Online ISBN: 978-1-59259-580-8

  • eBook Packages: Springer Protocols

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