Abstract
Recent extensive research on cytochrome P450 (P450) has clarified that P450 constitutes a superfamily of hemoproteins, which are believed to have evolved from a common ancestor. The versatility of P450 is mainly owing to the existence of a large number of P450 species, each of which shows a broad but somewhat unique substrate specificity.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Yabusaki, Y., Shimizu, M, Murakami, H, Nakamura, K, Oeda, K., and Ohkawa, H. (1984) Nucleotide sequence of a full-length cDNA coding for 3-methylcholanthrene-mduced rat liver cytochrome P-450MC Nucleic Acids Res. 12, 2929–2938.
Yabusaki, Y, Murakami, H., and Ohkawa, H (1988) Primary structure of Saccharomyces cerevisiae NADPH-cytochrome P450 reductase deduced from nucle-otide sequence of its cloned gene. J Biochem. 103, 1004–1010.
Oeda, K., Sakaki, T., and Ohkawa, H. (1985) Expression of rat liver cytochrome P-450MC cDNA in Saccharomyces cerevisiae. DNA 4, 203–210.
Murakami, H., Yabusaki, Y, Sakaki, T., Shibata, M, and Ohkawa, H (1990) Expression of cloned yeast NADPH-cytochrome P450 reductase gene in Saccha-romyces cerevisiae. J Biochem 108, 859–865
Sakaki, T., Kominami, S., Takemori, S., Ohkawa, H., Akiyoshi-Shibata, M., and Yabusaki, Y. (1994) Kinetic studies on a genetically engineered fused enzyme between rat cytochrome P450IAl and yeast NADPH-P450 reductase Biochemis-try 33, 4933–4939.
Yabusaki, Y (1995) Artificial P450/reductase fusion enzymes: what can we learn from their structures? Biochimie 77, 594–603.
Guengerich, F. P., Brian, W. R, Sari, M. A., and Ross, J T (1991) Expression of mammalian cytochrome P450 enzymes using yeast-based vectors Methods Enzymol. 206, 130–145.
Sakaki, T., Shibata, M., Yabusaki, Y, Murakami, H, and Ohkawa, H. (1989) Expression of bovine cytochrome P450c17 cDNA in Saccharomyces cerevisiae DNA 8, 409–418
Murakami, H, Yabusaki, Y, and Ohkawa, H. (1986) Expression of rat NADPH-cytochrome P-450 reductase cDNA in Saccharomyces cerevwae. DNA 5, 1–10
Eugster, H. P., Bartsch, S., Wurgler, F. E, and Sengstag, C (1992) Functional co-expression of human oxidoreductase and cytochrome P4501Al in Saccharomyces cerevisiae results in increased EROD activity. Biochem. Biophys. Res. Commun. 185, 641–647
Urban, P., Truan, G., Galtier, J. C., and Pompon, D. (1993) Xenobiotic metabo-lism in humamzed yeast: Engineered yeast cells producing human NADPH-cyto-chrome P450 reductase, cytochrome b5, epoxide hydrolase and P450s. Biochem. Sot. Trans 21, 1028–1034.
Sakaki, T., Shibata, M, Yabusaki, Y, Murakami, H., and Ohkawa, H. (1990) Expression of bovine cytochrome P450c21 and Its fused enzyme with yeast NADPH-cytochrome P450 reductase in Saccharomyces cerevisiae DNA Cell Biol 9, 603–614
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc.
About this protocol
Cite this protocol
Yabusaki, Y. (1998). Expression of Mammalian Cytochromes P450 in Yeast. In: Phillips, I.R., Shephard, E.A. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology™, vol 107. Humana Press. https://doi.org/10.1385/0-89603-519-0:195
Download citation
DOI: https://doi.org/10.1385/0-89603-519-0:195
Publisher Name: Humana Press
Print ISBN: 978-0-89603-519-5
Online ISBN: 978-1-59259-580-8
eBook Packages: Springer Protocols