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Microarrays in Inflammation

  • Andreas Bosio
  • Bernhard Gerstmayer

Part of the Progress in Inflammation Research book series (PIR)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Microarray and inflammation: An introduction

  3. Methods and protocols for the generation of gene expression profiles in inflammation research

    1. Front Matter
      Pages 13-13
    2. Birgit Sawitzki, Hans-Dieter Volk
      Pages 15-30
    3. Andreas Grützkau, Andreas Radbruch
      Pages 31-40
    4. Olaf Holtkötter, Dirk Petersohn
      Pages 41-50
    5. Nathalie Viguerie
      Pages 51-58
    6. Victor Appay, Martin Larsen
      Pages 59-70
    7. Jürgen Schmitz
      Pages 71-80
    8. Single and rare cell analysis — amplification methods

      1. Stephen D. Ginsberg
        Pages 81-94
  4. Selected applications of microarrays in inflammation research

    1. Front Matter
      Pages 105-105
    2. Kenji Izuhara, Sachiko Kanaji, Shoichiro Ohta, Hiroshi Shiraishi, Kazuhiko Arima, Noriko Yuyama
      Pages 107-114
    3. Enrico Tiacci, Verena Brune, Ralf Küppers
      Pages 131-144
    4. Thomas Häupl, Andreas Grützkau, Bruno Stuhlmüller, Karl Skriner, Gerd Burmester, Andreas Radbruch
      Pages 145-160
    5. Astrid Novosel, Arndt Borkhardt
      Pages 161-177
    6. Cross-platform microarray analysis

    7. A regulatory perspective

      1. Roland Frötschl, Peter Kasper
        Pages 199-209
  5. Outlook

    1. Front Matter
      Pages 211-211
    2. Bernhard Gerstmayer
      Pages 213-224
  6. Back Matter
    Pages 225-234

About this book

Introduction

The book is of interest to academic and industrial researchers but also clinicians who are working on inflammation related topics and would like to extend their knowledge about microarrays and the application thereof. After reading the book a researcher or clinician should be in the position to plan, perform and analyse or to critically review microarray experiments related to inflammation research.

So far, non of the published books covers all aspects which need to be taken into account when planning and performing a microrray experiment in inflammation research. Normally, for a researcher or clinician doing a microarray experiment it is not enough to have knowledge about microarrays , as the experiment does not start with purified and quality controlled RNA and does not end with signal intensities. Things like appropriate cell sorting techniques, stabilisation and transport of RNA or cells, the knowledge how to deal with whole blood as well as options for automation are crucial for a successful application of microarrays. Moreover these aspects are the main obstacles for transferring microarray based inflammation analysis from bench to bedside and the limiting factors for higher through put as needed in multicenter studies.

Keywords

Expression PCR Polymerasekettenreaktion RNA asthma blood cell gene gene expression genes inflammation lymphoma microarray research tissue

Editors and affiliations

  • Andreas Bosio
    • 1
  • Bernhard Gerstmayer
    • 2
  1. 1.Research & DevelopmentMiltenyi Biotec GmbHGladbachGermany
  2. 2.Miltenyi Biotec GmbHGladbachGermany

Bibliographic information

Industry Sectors
Pharma
Biotechnology