Despite inherent difficulties, the use of technologies in Omic such as DNA microarrays, that allow a comparison of global expression changes in thousands of genes between different conditions, appears to be a useful tool to study the influence of nutrition and gene-environment interactions in complex pathologies such as obesity, and thus to advance research.
The purpose of the present review is to provide some advice for fat specimen collection and RNA preparation in view of gene expression profiling analysis. Our data indicate that the use of RNA-stabilization reagents is not necessary. Isolated or cultured cells should be stored frozen in lysis buffer. Isolated adipose cells or pieces of adipose tissue must be shock-frozen in liquid nitrogen before storage. Total RNA extraction can then be performed using optimized tissue lipid RNA extraction systems. For clinical projects, fat biopsies of 0.5 g are recommended. Total RNA should be extracted using a modified tissue lipid extraction kit. Finally, some selected examples will show how previous transcriptomic approaches to human adipose tissue biopsies have contributed to advancing knowledge in the field of obesity, as much at the cellular as at the tissue level.
KeywordsAdipose Tissue Tissue Lipid Human Adipose Tissue Expression Profile Analysis Transcriptomic Approach
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