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Samples isolated by flow cytometry

  • Victor Appay
  • Martin Larsen
Part of the Progress in Inflammation Research book series (PIR)

Abstract

In recent years, important technological developments have provided new means to study fundamental aspects in biology and pathology. The emergence of DNA microarray technology enables us to analyze the expression levels of thousands of genes within cells, and to explore the underlying genetic causes of many human diseases. Moreover, the development of global PCR techniques now enables 1 to 10 million fold mRNA amplifications, and therefore microarray analysis from limited biological materials. Fluorescence Activated Cell Sorting (FACS) offers us the opportunity to separate cell populations according to their phenotypic or physical attributes, including rare cell types from heterogeneous mixtures, and to purify these for further characterization. In this chapter we discuss the possibility of combining these major technological advances in order to perform gene expression profiling on isolated cells of interest. The synergy of FACS and microarray technologies is bound to further our capacity to unveil the secrets of life.

Keywords

Fluorescence Activate Cell Sort Perform Gene Expression Density Gradient Separation mRNA Amplification Subset Accord 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag Basel/Switzerland 2008

Authors and Affiliations

  • Victor Appay
    • 1
  • Martin Larsen
    • 1
  1. 1.Immunologie Cellulaire et Tissulaire, INSERM U543, Faculté de Mé decineHôpital PitiéSalpêtrièreParis Cedex 13France

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