Abstract
In recent years, important technological developments have provided new means to study fundamental aspects in biology and pathology. The emergence of DNA microarray technology enables us to analyze the expression levels of thousands of genes within cells, and to explore the underlying genetic causes of many human diseases. Moreover, the development of global PCR techniques now enables 1 to 10 million fold mRNA amplifications, and therefore microarray analysis from limited biological materials. Fluorescence Activated Cell Sorting (FACS) offers us the opportunity to separate cell populations according to their phenotypic or physical attributes, including rare cell types from heterogeneous mixtures, and to purify these for further characterization. In this chapter we discuss the possibility of combining these major technological advances in order to perform gene expression profiling on isolated cells of interest. The synergy of FACS and microarray technologies is bound to further our capacity to unveil the secrets of life.
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Appay, V., Larsen, M. (2008). Samples isolated by flow cytometry. In: Bosio, A., Gerstmayer, B. (eds) Microarrays in Inflammation. Progress in Inflammation Research. Birkhäuser Basel. https://doi.org/10.1007/978-3-7643-8334-3_6
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DOI: https://doi.org/10.1007/978-3-7643-8334-3_6
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