Skin and skin models

  • Olaf Holtkötter
  • Dirk Petersohn
Part of the Progress in Inflammation Research book series (PIR)


Skin inflammation is an often-occurring phenomenon in a wide range of skin pathologies. In order to understand the basics of the underlying molecular mechanisms, early responses such as gene expression changes in this tissue are the focus of numerous studies. The prerequisite for gene expression analysis is the isolation of relevant tissue samples and the subsequent preparation of high quality RNA.

Different sources of tissue samples (e.g. skin biopsies, suction blister, epidermis models and bio-engineered full thickness skin), their benefits and disadvantages are discussed. Furthermore, we briefly describe ways of isolating RNA and assessing its quality, which is essential for performing successful gene expression studies.


Skin Inflammation Gene Expression Change Gene Expression Study Full Thickness Thickness Skin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Thody AJ, Shuster S (1989) Control and function of sebaceous glands. Physiol Rev 69: 383–416PubMedGoogle Scholar
  2. 2.
    Steward ME, Downing DT (1991) Chemistry and function of mammalian sebaceous lipids. In: PM Elias (ed): Advances in Lipid Research, Vol. 24, Skin Lipids, San Diego Academic Press, San Diego, 263–302Google Scholar
  3. 3.
    Zouboulis CC, Eady A, Philpott M, Goldsmith LA, Orfanos C, Cunliffe WC, Rosenfield R(2005) What is the pathogenesis of acne? Exp Dermatol 14: 143–52PubMedCrossRefGoogle Scholar
  4. 4.
    Lin JY, Fisher DE (2007) Melanocyte biology and skin pigmentation. Nature 445: 843–50PubMedCrossRefGoogle Scholar
  5. 5.
    Schröder JM, Reich K, Kabashima K, Liu FT, Romani N, Metz M, Kerstan A, Lee PHGoogle Scholar
  6. Loser.
    K, Schön MP, Maurer M, Stoitzner P, Beissert S, Tokura Y, Gallo RL(2006) Who is really in control of skin immunity under physiological circumstances — lymphocytes, dendritic cells or keratinocytes? Exp Dermatol 15: 913–29Google Scholar
  7. 6.
    Bell E, Ivarsson B, Merrill C (1979) Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA 76: 1274–8PubMedCrossRefGoogle Scholar
  8. 7.
    Welss T, Basketter DA, Schröder KR (2004) In vitro skin irritation: facts and future. State of the art review of mechanisms and models. Toxicology in Vitro 8: 231–243CrossRefGoogle Scholar
  9. 8.
    Hochberg M, Zeligson S, Amariglio N, Rechavi G, Ingber A, Enk CD (2007) Genomic-scale analysis of psoriatic skin reveals differentially expressed insulin-like growth factorbinding protein-7 after phototherapy. Br J Dermatol 156: 289–300PubMedCrossRefGoogle Scholar
  10. 9.
    Wong R, Tran V, Morhenn V, Hung SP, Andersen B, Ito E, Wesley Hatfield G, Benson NR(2004) Use of RT-PCR and DNA microarrays to characterize RNA recovered by non-invasive tape harvesting of normal and inflamed skin.J Invest Dermatol 123: 159–67PubMedCrossRefGoogle Scholar
  11. 10.
    Wong R, Tran V, Talwalker S, Benson NR (2006) Analysis of RNA recovery and gene expression in the epidermis using non-invasive tape stripping. J Dermatol Sci 44: 81–92PubMedCrossRefGoogle Scholar
  12. 11.
    Welss T, Matthies W, Schroeder KR (2007) Compatibility testing in vitro: a comparison with in vivo patch test data. Int J Cosm Sci 29: 143CrossRefGoogle Scholar
  13. 12.
    Trost A, Bauer JW, Lanschutzer C, Laimer M, Emberger M, Hintner H, Onder K (2007) Rapid, high-quality and epidermal-specific isolation of RNA from human skin. Exp Dermatol 16: 185–90PubMedCrossRefGoogle Scholar
  14. 13.
    Black AF, Bouez C, Perrier E, Schlotmann K, Chapuis F, Damour O (2005) Optimization and characterization of an engineered human skin equivalent. Tissue Eng 11: 723–33PubMedCrossRefGoogle Scholar
  15. 14.
    Shahabeddin L, Berthod F, Damour O, Collombel C(1990) Characterization of skin reconstructed on a chitosan-cross-linked collagen-glycosaminoglycan matrix. Skin Pharmacol 3: 107–14PubMedCrossRefGoogle Scholar
  16. 15.
    Walzer C, Benathan M, Frenk E (1989) Thermolysin treatment: a new method for dermo-epidermal separation. J Invest Dermatol 92: 78–81PubMedCrossRefGoogle Scholar
  17. 16.
    Becker B, Roesch A, Hafner C, Stolz W, Dugas M, Landthaler M, Vogt T (2004) Discrimination of melanocytic tumors by cDNA array hybridization of tissues prepared by laser pressure catapulting. J Invest Dermatol 122: 361–8PubMedCrossRefGoogle Scholar
  18. 17.
    Nanney LB, Caldwell RL, Pollins AC, Cardwell NL, Opalenik SR, Davidson JM (2006) Novel approaches for understanding the mechanisms of wound repair. J Investig Dermatol Symp Proc 11: 132–9PubMedCrossRefGoogle Scholar
  19. 18.
    Sambrook J, Fritsch EF, Maniatis T(eds) (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  20. 19.
    Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Struhl K, Smith JA (eds) (2007) Current Protocols in Molecular Biology. John Wiley & SonGoogle Scholar
  21. 20.
    Mewes KR, Raus M, Bernd A, Zoller NN, Sattler A, Graf R (2007) Elastin expression in a newly developed full-thickness skin equivalent. Skin Pharmacol Physiol 20: 85–95PubMedCrossRefGoogle Scholar

Copyright information

© Birkhäuser Verlag Basel/Switzerland 2008

Authors and Affiliations

  • Olaf Holtkötter
    • 1
  • Dirk Petersohn
    • 1
  1. 1.Phenion GmbH & Co. KGDüsseldorfGermany

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