Skin and skin models
Skin inflammation is an often-occurring phenomenon in a wide range of skin pathologies. In order to understand the basics of the underlying molecular mechanisms, early responses such as gene expression changes in this tissue are the focus of numerous studies. The prerequisite for gene expression analysis is the isolation of relevant tissue samples and the subsequent preparation of high quality RNA.
Different sources of tissue samples (e.g. skin biopsies, suction blister, epidermis models and bio-engineered full thickness skin), their benefits and disadvantages are discussed. Furthermore, we briefly describe ways of isolating RNA and assessing its quality, which is essential for performing successful gene expression studies.
KeywordsSkin Inflammation Gene Expression Change Gene Expression Study Full Thickness Thickness Skin
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