Microbial Production

From Genome Design to Cell Engineering

  • Hideharu Anazawa
  • Sakayu Shimizu

Table of contents

  1. Front Matter
    Pages i-ix
  2. Minimum Genome Factory

    1. Front Matter
      Pages 1-1
    2. Katsutoshi Ara, Kenji Manabe, Shenghao Liu, Yasushi Kageyama, Tadahiro Ozawa, Masatoshi Tohata et al.
      Pages 3-15
    3. Hiromichi Kumagai, Mayumi Sasaki, Alimjan Idiris, Hideki Tohda
      Pages 17-24
  3. Whole Genome Manipulation for Genome Design

    1. Front Matter
      Pages 33-33
    2. Kiyoko T. Miyamoto, Haruo Ikeda
      Pages 63-72
  4. Application of Omics Information and Construction of Mutant Libraries

    1. Front Matter
      Pages 73-73
    2. Myco Umemura, Masayuki Machida
      Pages 75-85
    3. Hirotada Mori, Rikiya Takeuchi, Yuta Otsuka, Yong Han Tek, Wataru Nomura, Barry L. Wanner
      Pages 97-108
  5. Applications of Advanced Technologies for Production

    1. Front Matter
      Pages 119-119
    2. Akira Iwasaki, Noriyuki Ito, Yoshihiko Yasohara
      Pages 121-129
    3. Takeko Kodama, Kenji Manabe, Katsutoshi Ara, Junichi Sekiguchi
      Pages 141-147

About this book


In this book the reader will find an introduction and review of recent research and development of the technology for bio-production of manufacturing microorganisms, a field in which Japan leads the world. Presented here are novel concepts and technology for breeding fermentation strains as well as the analytical methodology for gene function and genome design of producer strains.

Successful results are shown in diverse fields of application such as microbiological production, pharmaceuticals, chemicals, foods, and cosmetics. Two methodologies announced in the twenty-first century have dramatically improved the productivity of fermentation strains. One is a method of synthesizing the entire genome of the fermentation strain as proposed by Venter, famous for his work in human genome sequencing. Another is the minimum genome factory, which provides the basis for breeding a strain whose genome optimizes production of the target material.

Each chapter has been written by specialists in academia or by active researchers in industry, creating a work that is informative for researchers interested in the industrialization of novel concepts or technologies by a microbial production system. The book consists of three parts. The first presents the innovative concepts and technology for improvement of the fermentation strain, with supporting technology and other information. The second describes novel technologies useful for analysis of cell activities, and the third provides practical applications of those technologies and success stories for microbial production.


Minimum genome factory Molecular breeding Omics information bio-production cell activities fermentation genome manipulation microorganism

Editors and affiliations

  • Hideharu Anazawa
    • 1
  • Sakayu Shimizu
    • 2
  1. 1.Japan Bioindustry AssociationChuo-ku, TokyoJapan
  2. 2.Bioscience and BiotechnologyGraduate School of Environmental Science, Kyoto Gakuen UniversityKameokaJapan

Bibliographic information

  • DOI https://doi.org/10.1007/978-4-431-54607-8
  • Copyright Information Springer Japan 2014
  • Publisher Name Springer, Tokyo
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-4-431-54606-1
  • Online ISBN 978-4-431-54607-8
  • About this book