Abstract
The research program on the “minimum genome factory (MGF)” was launched in 2001. In this program, several microorganisms have been genetically modified to breed a cell with fewer genes on chromosomes of reduced size and expected less regulation on the gene network to be an ideal platform for a cell-factory. The genome of Escherichia coli has been reduced to 3.6 Mbp, by elimination of a total 1.06-Mbp region on various sites. This reduced-genome strain, MGF-01, has been grown to reach a higher level in minimal medium. This strain has the potential to be a general host for recombinant cells with higher productivity by retaining robustness of regulation on the metabolic network and activation of the fundamental biosynthetic pathway.
Using MGF-01 as a host strain, the production of amino acid and ATP are elevated to several fold.
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References
Baba T et al (2006) Construction of E. coli K-12 in-frame single-gene knock out mutants: the Keio collection. Mol Syst Biol 2:20060008
Fukiya S et al (2004a) Extensive diversity in pathogenic E. coli and Shigella strains revealed by comparative genomic hybridization microarray. J Bacteriol 186(2):3911–3921
Fukiya S et al (2004b) An improved method for deleting large regions of E.coli K-12 chromosome using a combination of Cre/loxP and λRed. FEMS Microbiol Lett 234:325–331
Hara KY et al (2009) Systematic genome-wide scanning for genes involved in ATP generation. Metab Eng 11:1–7
Hirokawa Y et al (2013) Genetic manipulation restored the growth fitness or reduced- genome E. coli. J Biosci Bioeng 116(1):52–58
Ito M et al (2005) Functional analysis of 1440 E. coli genes using the combination of knock-out library and phenotype microarray. Metab Eng 7:318–327
Mizoguchi et al (2006) WO2006/057340
Mizoguchi H, Mori H, Fujio T (2007a) Escherichia coli minimum genome factory. Biotechnol Appl Biochem 46(pt 3):157–167
Mizoguchi H et al (2007b) A simple method for multiple modification of E. coli K-12 chromosome. Biosci Biotechnol Biochem 71(12):2905–2911
Mizoguchi H et al (2008) Superpositioning of deletions promotes growth of E. coli with reduced genome. DNA Res 15:277–284
Posfai G et al (2006) Emergent properties of reduced genome E. coli. Science 312:1044–1046
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Anazawa, H. (2014). The Concept of the Escherichia coli Minimum Genome Factory. In: Anazawa, H., Shimizu, S. (eds) Microbial Production. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54607-8_3
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DOI: https://doi.org/10.1007/978-4-431-54607-8_3
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