Skip to main content
SpringerLink
Log in

Genome Design of Actinomycetes for Secondary Metabolism

  • Chapter
  • First Online:
Microbial Production

  • 2036 Accesses

Abstract

An industrial microorganism, Streptomyces avermitilis, which is the producer of the anthelmintic agent avermectin, has been constructed as a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis. Several kinds of the entire biosynthetic gene clusters for secondary metabolites were successively cloned and introduced into a versatile model host, S. avermitilis. Most S. avermitilis transformants carrying the entire gene cluster produced metabolite(s) caused by the expression of the introduced biosynthetic gene clusters. Productivity of the metabolite(s) in some transformants of the versatile host was higher than that of the original producers.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Barbe V et al (2011) Complete genome sequence of Streptomyces cattleya NRRL 8057, a producer of antibiotics and fluorometabolites. J Bacteriol 193:5055–5056

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Bentley SD et al (2002) Complete genome sequence of model of actinomycete Streptomyces coelicolor A3(2). Nature (Lond) 417:141–147

    Article  Google Scholar 

  • Bignell DR et al (2010) Streptomyces scabies 87–22 contains a coronafacic acid-like biosynthetic cluster that contributes to plant-microbe interactions. Mol Plant Microbe Interact 23:161–175

    Article  CAS  PubMed  Google Scholar 

  • Broach JR, Hichs JB (1980) Replication and recombination functions associated with the yeast plasmid, 2μ circle. Cell 21:501–508

    Article  CAS  PubMed  Google Scholar 

  • Burg RW et al (1979) Avermectins, new family of potent anthelminthic agents: producing organism and fermentation. Antimicrob Agents Chemother 15:361–367

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Cole ST et al (1998) Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature (Lond) 393:537–544

    Article  CAS  Google Scholar 

  • Gomez-Escribano JB, Bibb MJ (2011) Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters. Microb Biotechnol 4:207–215

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hung CH et al (1998) The telomeres of Streptomyces chromosomes contain conserved palindromic sequences with potential to form complex secondary structures. Mol Microbiol 28:905–916

    Article  Google Scholar 

  • Ichikawa N et al (2010) Genome sequence of Kitasatospora setae NBRC 14216T: an evolutionary snapshot of the family Streptomycetaceae. DNA Res 17:393–406

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ikeda H et al (2003) Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. Nat Biotechnol 21:526–531

    Article  PubMed  Google Scholar 

  • Kitani S et al (2011) Avenolide, a Streptomyces hormone controlling antibiotic production in Streptomyces avermitilis. Proc Natl Acad Sci USA 108:16410–16415

    Article  CAS  PubMed  Google Scholar 

  • Komatsu M et al (2008) Identification and functional analysis of genes controlling biosynthesis of 2-methylisoborneol. Proc Natl Acad Sci USA 105:7422–7427

    Article  CAS  PubMed  Google Scholar 

  • Komatsu M et al (2010) Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc Natl Acad Sci USA 107:2646–2651

    Article  CAS  PubMed  Google Scholar 

  • Komatsu M et al (2013) Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites. ACS Synth Biol 2:384–396

    Google Scholar 

  • McLeod MP et al (2006) The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proc Natl Acad Sci USA 103:15582–15587

    Article  PubMed  Google Scholar 

  • Medema MH et al (2010) The sequence of a 1.8-Mb bacterial linear plasmid reveals a rich evolutionary reservoir of secondary metabolic pathways. Genome Biol Evol 2:212–224

    Article  PubMed Central  PubMed  Google Scholar 

  • Ohnishi Y et al (2008) Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350. J Bacteriol 190:4050–4060

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Omura S et al (2001) Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. Proc Natl Acad Sci USA 98:12215–12220

    Article  CAS  PubMed  Google Scholar 

  • Pullan S et al (2011) Genome-wide analysis of the role of GlnR in Streptomyces venezuelae provides new insights into global nitrogen regulation in actinomycetes. BMC Genomics 12:175

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Strenberg N et al (1981) Bacteriophage P1 site-specific recombination. II. Recombination between loxP and the bacterial chromosome. J Mol Biol 150:487–507

    Article  Google Scholar 

  • Tanaka Y et al (2009) Antibiotic overproduction by rpsL and rsmG mutants of various actinomycetes. Appl Environ Microbiol 75:4919–4922

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Waksman SA (1953) Streptomycin: background, isolation, properties, and utilization. Science 118:259–266

    Article  CAS  PubMed  Google Scholar 

  • Wang XJ et al (2010) Genome sequence of the milbemycin-producing bacterium Streptomyces bingchenggensis. J Bacteriol 192:4526–4527

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kitasato Institute for Life Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan

    Kiyoko T. Miyamoto & Haruo Ikeda

Authors
  1. Kiyoko T. Miyamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Haruo Ikeda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Haruo Ikeda .

Editor information

Editors and Affiliations

  1. Japan Bioindustry Association, Chuo-ku, Tokyo, Tokyo, Japan

    Hideharu Anazawa

  2. Bioscience and Biotechnology, Graduate School of Environmental Science, Kyoto Gakuen University, Kameoka, Kyoto, Japan

    Sakayu Shimizu

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Japan

About this chapter

Cite this chapter

Miyamoto, K.T., Ikeda, H. (2014). Genome Design of Actinomycetes for Secondary Metabolism. In: Anazawa, H., Shimizu, S. (eds) Microbial Production. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54607-8_6

Download citation

Publish with us

Policies and ethics

Search

Navigation