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  • Book
  • © 2009

Nucleic Acid and Peptide Aptamers

Methods and Protocols

Editors:

  • Provides an easily accessible reference volume for in vitro selection procedures written by leading researchers in the field

  • Covers a wide range of in vitro selection protocols targeting different molecules

  • Comprehensive guide to identifying your specific target binding aptamer, whether of nucleic acid or peptidic nature

  • Detailed presentation of applications of aptamers

  • The first comprehensive summary that covers peptide and nucleic acid aptamers in one volume

  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 535)

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eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-59745-557-2
  • Instant PDF download
  • Readable on all devices
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  • Tax calculation will be finalised during checkout

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
  2. Part I Nucleic Acid Aptamers

    1. Front Matter

      Pages 1-1
    2. Nucleic Acid Pool Preparation and Characterization

      • Shawn K. Piasecki, Bradley Hall, Andrew D. Ellington
      Pages 3-18
    3. Isolating Aptamers Using Capillary Electrophoresis–SELEX (CE–SELEX)

      • Renee K. Mosing, Michael T. Bowser
      Pages 33-43
    4. In Vitro Selection of Allosteric Ribozymes

      • Nicolas Piganeau
      Pages 45-57
    5. Cell-Specific Aptamers for Targeted Therapies

      • Laura Cerchia, Paloma H. Giangrande, James O. McNamara, Vittorio de Franciscis
      Pages 59-78
    6. Aptamers Targeting RNA Molecules

      • Marguerite Watrin, Eric Dausse, Isabelle Lebars, Bernard Rayner, Anthony Bugaut, Jean-Jacques Toulmé
      Pages 79-105
    7. Structural Probing Techniques on Natural Aptamers

      • Catherine A. Wakeman, Wade C. Winkler
      Pages 115-133
    8. Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography

      • Andrea L. Edwards, Andrew D. Garst, Robert T. Batey
      Pages 135-163
    9. Locked Nucleic Acid Aptamers

      • Jan Barciszewski, Michael Medgaard, Troels Koch, Jens Kurreck, Volker A. Erdmann
      Pages 165-186
    10. Aptamers as Artificial Gene Regulation Elements

      • Beatrix Suess, Julia E. Weigand
      Pages 201-208
    11. Aptamers and Biosensors

      • Thomas M. A. Gronewold
      Pages 209-222
    12. Nanoparticles/Dip Stick

      • Yi Lu, Juewen Liu, Debapriya Mazumdar
      Pages 223-239
    13. In Vivo Imaging of Oligonucleotidic Aptamers

      • Bertrand Tavitian, Frédéric Ducongé, Raphaël Boisgard, Frédéric Dollé
      Pages 241-259
  3. Part II Peptide Aptamers

    1. Front Matter

      Pages 1-1
    2. Selection of Peptides Interfering with Protein–Protein Interaction

      • Annette Gaida, Urs B. Hagemann, Dinah Mattay, Christina Räuber, Kristian M. Müller, Katja M. Arndt
      Pages 263-291
    3. In Vitro Selection of Protein and Peptide Libraries Using mRNA Display

      • Terry T. Takahashi, Richard W. Roberts
      Pages 293-314

About this book

After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In Nucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.

Keywords

  • Ligands
  • Protein modification
  • RNA
  • Target molecules
  • genes
  • in vitro methods
  • molecular biology

Reviews

From the reviews:

"The book ‘Nucleic Acids and Peptide Aptamers’ is a collection of detailed protocols describing various aspects related to generating aptamers, characterizing them, and applying for particular purposes. … The list of references is a useful and timely source of further reading on the subject. … can be certainly recommended as an excellent handbook addressed to researchers and students in the molecular biology field." (Ryszard W. Adamiak and Mikolaj Olejniczak, Acta Biochimica Polonica, Vol. 56 (3), 2009)

Bibliographic Information

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-59745-557-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout