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Cell-Specific Aptamers for Targeted Therapies

  • Laura Cerchia
  • Paloma H. Giangrande
  • James O. McNamara
  • Vittorio de Franciscis
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 535)

Abstract

Many signalling proteins involved in diverse functions such as cell growth and differentiation can act as oncogenes and cause cellular transformation. These molecules represent attractive targets for cancer diagnosis or therapy and therefore are subject to intensive investigation.

Aptamers are small, highly structured nucleic acid molecules, isolated from combinatorial libraries by a procedure termed SELEX. Aptamers bind to a target molecule by providing a limited number of specific contact points imbedded in a larger, defined three-dimensional structure. Recently, aptamers have been selected against whole living cells, opening a new path which presents three major advantages: (1) direct selection without prior purification of membrane-bound targets, (2) access to membrane proteins in their native conformation similar to the in vivo conditions and (3) identification of (new) targets related to a specific phenotype. The ability to raise aptamers against living cells opens some attractive possibilities for new therapeutic and delivery approaches. In this chapter, the most recent advances in the field will be reviewed together with detailed descriptions of the relevant experimental approaches.

Key words

Aptamer SELEX ret delivery siRNA 

Notes

Acknowledgements

This work was supported by the European Molecular Imaging Laboratory (EMIL) Network (LSHC-2004-503569) and by the MIUR-FIRB Grant (#RBIN04J4J7).

We wish to thank C.L. Esposito, B. Tavitian, F. Duconge and D. Libri for fruitful discussions.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Laura Cerchia
    • 1
  • Paloma H. Giangrande
    • 2
  • James O. McNamara
    • 2
  • Vittorio de Franciscis
    • 1
  1. 1.Istituto per l’Endocrinologia e Oncologia Sperimentale “G. Salvatore,”Italy
  2. 2.Department of Internal Medicine, Division of CardiologyUniversity of IowaIowa CityUSA

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