Locked Nucleic Acid Aptamers

  • Jan Barciszewski
  • Michael Medgaard
  • Troels Koch
  • Jens Kurreck
  • Volker A. Erdmann
Part of the Methods in Molecular Biology™ book series (MIMB, volume 535)


The aptamer technology has been introduced in the early 1990s. With this technique ligands for organic dyes and proteins have been identified in many research field, providing various inhibitory molecules that allow functional interference in biological systems. Aptamers can therefore be employed for various applications ranging from diagnostic to therapeutic assay formats. Locked nucleic acid aptamers (LNA-Aps) are oligonucleotides containing one or more LNA nucleotide monomers with a bicyclic furanose unit locked in an RNA mimicking sugar conformation, evolved in vitro to bind target ligands with high affinity and specificity. LNA-Aps are attractive alternatives to antibody- and small-molecule-based therapeutics due to their stability, low toxicity and immunogenecity.

Key words

LNA – locked nucleic acid LNA-As – LNA antisense LNA-Ag – LNA antigene LNA-Ap – LNA aptamer 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jan Barciszewski
    • 1
  • Michael Medgaard
    • 2
  • Troels Koch
    • 3
  • Jens Kurreck
    • 4
  • Volker A. Erdmann
    • 5
  1. 1.Institute of Bioorganic Chemistry of the Polish Academy of SciencesPoland
  2. 2.Santaris PharmaDenmark
  3. 3.Santaris PharmaDenmark
  4. 4.Institute for Chemistry and BiochemistryFree University Berlin, Berlin, Germany; Institute of Industrial Genetics, University of StuttgartGermany
  5. 5.Institute for Chemistry and BiochemistryFree University BerlinGermany

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