Abstract
In vitro selection techniques offer powerful and versatile methods to isolate nucleic acid sequences with specific activities from huge libraries. The present protocol describes an in vitro selection strategy for the de novo selection of allosteric self-cleaving ribozymes responding to virtually any drug of choice. We applied this method to select hammerhead ribozymes inhibited specifically by doxycycline or pefloxacin in the sub-micromolar range. The selected ribozymes can be converted into classical aptamers via insertion of a point mutation in the catalytic center of the ribozyme.
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References
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Piganeau, N. (2009). In Vitro Selection of Allosteric Ribozymes. In: Mayer, G. (eds) Nucleic Acid and Peptide Aptamers. Methods in Molecular Biology™, vol 535. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-557-2_4
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DOI: https://doi.org/10.1007/978-1-59745-557-2_4
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-934115-89-3
Online ISBN: 978-1-59745-557-2
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