Abstract
In vitro genetic approaches are powerful solutions to the protein design problem. mRNA display is an in vitro selection technique enabling the design of peptide and protein ligands ranging from one to over 100 amino acids. Libraries containing more than 10 trillion unique sequences can be synthesized and sieved with exquisite control over binding stringency and specificity.
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Acknowledgments
We would like to thank Dr. Ryan Austin, Stephen Fiacco, and Shannon Howell for helpful comments on the manuscript. This work supported by NIH grant NIH GM R01 60416 and NIH GM R21 76678.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Takahashi, T.T., Roberts, R.W. (2009). In Vitro Selection of Protein and Peptide Libraries Using mRNA Display. In: Mayer, G. (eds) Nucleic Acid and Peptide Aptamers. Methods in Molecular Biology™, vol 535. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-557-2_17
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DOI: https://doi.org/10.1007/978-1-59745-557-2_17
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