Skip to main content
SpringerLink
Log in

Prion Protein Protocols

  • Book
  • © 2008

Overview

Editors:
  1. Andrew F. Hill

    1. Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute; Department of Pathology and Mental Health Institute of Victoria, University of Melbourne, Melbourne, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Most current collection of protocols in prion disease research
  • Fully described methods chapters from leaders in the field with easy to follow instructions and notes sections
  • Provides methods for studying prion infection in cell cultures with a review chapter on current cell models
  • Detailed protocols for prion protein conversion assays using current technologies with a review highlighting their use
  • Comprehensive chapter on the molecular diagnosis of human prion diseases including full methods
  • Reviews of the use of transgenic mouse models of prion diseases
  • Methods for the biochemical characterization of mammalian prion proteins

Part of the book series: Methods in Molecular Biology (MIMB, volume 459)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Cell Culture Models to Unravel Prion Protein Function and Aberrancies in Prion Diseases

    • Katarina Bedecs
    Pages 1-20

  3. Investigation of PrPC Metabolism and Function in Live Cells

    • Cathryn L. Haigh, David R. Brown
    Pages 21-34

  4. Immunodetection of PrPSc Using Western and Slot Blotting Techniques

    • Hanna Gyllberg, Kajsa Löfgren
    Pages 35-48

  5. Assaying Prions in Cell Culture

    • Sukhvir P. Mahal, Cheryl A. Demczyk, Emery W. Smith Jr., Peter-Christian Klohn, Charles Weissmann
    Pages 49-68

  6. Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes

    • Laura J. Vella, Andrew F. Hill
    Pages 69-82

  7. Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons

    • Giuseppe D. Ciccotosto, Roberto Cappai, Anthony R. White
    Pages 83-96

  8. Understanding the Nature of Prion Diseases Using Cell-free Assays

    • Victoria A. Lawson
    Pages 97-103

  9. Methods for Conversion of Prion Protein into Amyloid Fibrils

    • Leonid Breydo, Natallia Makarava, Ilia V. Baskakov
    Pages 105-115

  10. Amplification of Purified Prions In Vitro

    • Surachai Supattapone, Nathan R. Deleault, Judy R. Rees
    Pages 117-130

  11. Expression and Purification of Full-Length Recombinant PrP of High Purity

    • Natallia Makarava, Ilia V. Baskakov
    Pages 131-143

  12. Analysis of PrP Conformation Using Circular Dichroism

    • Sen Han, Andrew F. Hill
    Pages 145-159

  13. Effect of Copper on the De Novo Generation of Prion Protein Expressed in Pichia pastoris

    • Carina Treiber
    Pages 161-172

  14. Biophysical Investigations of the Prion Protein Using Electron Paramagnetic Resonance

    • Simon C. Drew, Kevin J. Barnham
    Pages 173-196

  15. Molecular Diagnosis of Human Prion Disease

    • Jonathan D. F. Wadsworth, Caroline Powell, Jonathan A. Beck, Susan Joiner, Jacqueline M. Linehan, Sebastian Brandner et al.
    Pages 197-227

  16. Analysis of Endogenous PrPC Processing in Neuronal and Non-neuronal Cell Lines

    • Victoria Lewis, Steven J. Collins
    Pages 229-239

  17. Molecular Typing of PrPres in Human Sporadic CJD Brain Tissue

    • Victoria Lewis, Genevieve M. Klug, Andrew F. Hill, Steven J. Collins
    Pages 241-247

  18. Transgenic Mouse Models of Prion Diseases

    • Glenn C. Telling
    Pages 249-263

  19. Quantitative Bioassay of Surface-bound Prion Infectivity

    • Victoria A. Lawson
    Pages 265-273

  20. Back Matter

    Pages 275-280
    Download chapter PDF
Back to top

Keywords

About this book

Prion Protein Protocols brings together a collection of current protocols in the field of mammalian prion disease research. Since identification of the prion protein gene some 20 years ago, what were once thought to be rare, neurodegenerative diseases of humans and animals have become a major research area. The major interest in this field results from the epidemic of bovine spongiform encephalopathy and the subsequent human prion disease, variant Creutzfeldt–Jakob disease, which still poses an unknown risk to human health. The “unknowns” in the field stem from the enigmatic nature of infectious prions, the infectious agent that can transmit these diseases between individuals. Although much information has been gained over the past two decades about the molecular nature of prion proteins, only very recently have several research groups begun to cast light on how to turn the normal prion protein into its aberrant, infectious form. Prion Protein Protocols brings together a collection of protocols from 13 diff- ent laboratories in five countries covering basic science and diagnostic areas of prion research. Together, the chapters provide an up-to-date collection of current methods in this unique area of neuroscience. The notes section at the end of each methods chapter provides useful insight into the experimental techniques, and they are no doubt a benefit to researchers wanting to use these technologies.

Reviews

From the reviews:

"Interspersed are concise reviews focusing on current growth areas in the prion field. … The protocols are clear and authoritative. For those entering the field it would be an excellent guide." (Hugh Pennington, Microbiology Today, February, 2009)

Editors and Affiliations

  • Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute; Department of Pathology and Mental Health Institute of Victoria, University of Melbourne, Melbourne, Australia

    Andrew F. Hill

Bibliographic Information

Publish with us

Policies and ethics

Back to top

Search

Navigation