Summary
Prion infectivity is often linked to presence of the protease-resistant isoform of prion protein (PrP), PrPres; therefore, it is of highest interest to have convenient methods for rapid detection of PrPres in the research laboratory. For detection of PrPres in model systems to confirm infectivity, there are several methods that can be applied. This chapter focuses on detection of PrPres by proteinase K digestion followed by Western blot, which is the only method that is both quantitative and qualitative. For large-scale screening of PrPres content in samples, the dot blot method offers a great advantage for detecting PrPres, and this method is also thoroughly described in this chapter.
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Gyllberg, H., Löfgren, K. (2008). Immunodetection of PrPSc Using Western and Slot Blotting Techniques. In: Hill, A.F. (eds) Prion Protein Protocols. Methods in Molecular Biology™, vol 459. Humana Press. https://doi.org/10.1007/978-1-59745-234-2_3
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DOI: https://doi.org/10.1007/978-1-59745-234-2_3
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