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Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes

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Book cover Prion Protein Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 459))

Summary

Prion-propagating cell lines are an efficient and useful means for studying the cellular and molecular mechanisms implicated in prion disease. Use of cell-based models has lead to the finding that prion protein (PrPC) and PrPSc are released from cells in association with exosomes. Furthermore, exosomes have been shown to act as vehicles for infectivity, transferring PrPSc between cell lines and providing a mechanism for prion spread between tissues. As a role for exo-somes in prion disease is emerging, this chapter outlines a method for the generation of prion-infected cell lines and the isolation and characterization of PrPC- and PrPSc-containing exosomes.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology and Department of Pathology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Melbourne, Australia

    Laura J. Vella & Andrew F. Hill

Authors
  1. Laura J. Vella
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  2. Andrew F. Hill
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Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute; Department of Pathology and Mental Health Institute of Victoria, University of Melbourne, 30 Flemington Road Parkville, Melbourne, VIC, 3010, Australia

    Andrew F. Hill

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© 2008 Humana Press, a part of Springer Science + Business Media, LLC

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Vella, L.J., Hill, A.F. (2008). Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes. In: Hill, A.F. (eds) Prion Protein Protocols. Methods in Molecular Biology™, vol 459. Humana Press. https://doi.org/10.1007/978-1-59745-234-2_5

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  • DOI: https://doi.org/10.1007/978-1-59745-234-2_5

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-897-3

  • Online ISBN: 978-1-59745-234-2

  • eBook Packages: Springer Protocols

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