Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1701)
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Table of contents (30 protocols)
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Construction of Antibody Phage Display Libraries
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Selection Strategies for Antibodies
Keywords
About this book
This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.
Editors and Affiliations
Bibliographic Information
Book Title: Phage Display
Book Subtitle: Methods and Protocols
Editors: Michael Hust, Theam Soon Lim
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7447-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2018
Hardcover ISBN: 978-1-4939-7446-7Published: 21 November 2017
Softcover ISBN: 978-1-4939-8488-6Published: 30 August 2018
eBook ISBN: 978-1-4939-7447-4Published: 08 November 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 562
Number of Illustrations: 17 b/w illustrations, 43 illustrations in colour
Topics: Immunology
Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma