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Phage Display pp 239–253Cite as

Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1701))

Abstract

Libraries of antibody fragments displayed on filamentous phages have proved their value to generate human antibodies against virtually any target. We describe here a simple protocol to make large and diverse libraries based on a single or a limited number of frameworks. The approach is flexible enough to be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab′)2), and to target in a single step the six complementarity-determining regions—or any other part—of the antibody molecule. Using this protocol, libraries larger than 1010 can be easily constructed in a single week.

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Author information

Authors and Affiliations

  1. IRCM, Institut de Recherche en Cancérologie de Montpellier, Montpellier, F-34298, France

    Déborah Caucheteur, Gautier Robin, Vincent Parez & Pierre Martineau

  2. INSERM, U1194, Montpellier, F-34298, France

    Déborah Caucheteur, Gautier Robin, Vincent Parez & Pierre Martineau

  3. Université de Montpellier, Montpellier, F-34090, France

    Déborah Caucheteur, Gautier Robin, Vincent Parez & Pierre Martineau

  4. Institut régional du Cancer de Montpellier, Montpellier, F-34298, France

    Déborah Caucheteur, Gautier Robin, Vincent Parez & Pierre Martineau

Authors
  1. Déborah Caucheteur
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  2. Gautier Robin
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  3. Vincent Parez
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  4. Pierre Martineau
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Corresponding author

Correspondence to Pierre Martineau .

Editor information

Editors and Affiliations

  1. Technische Universität Braunschweig, Braunschweig, Germany

    Michael Hust

  2. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia

    Theam Soon Lim

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Caucheteur, D., Robin, G., Parez, V., Martineau, P. (2018). Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies. In: Hust, M., Lim, T. (eds) Phage Display. Methods in Molecular Biology, vol 1701. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7447-4_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7447-4_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7446-7

  • Online ISBN: 978-1-4939-7447-4

  • eBook Packages: Springer Protocols

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