Developmental Hematopoiesis

Methods and Protocols

  • Margaret H. Baron

Part of the Methods in Molecular Medicine book series (MIMM, volume 105)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Genetic Approaches

  3. Analysis of Cell Fate Outcomes in Transplantation Models

    1. Front Matter
      Pages 63-63
    2. Edward F. Srour, Mervin C. Yoder
      Pages 65-79
    3. Elisabeth H. Javazon, Aziz M. Merchant, Enrico Danzer, Alan W. Flake
      Pages 81-94
  4. Animal Models

    1. Front Matter
      Pages 107-107
    2. Drosophila

      1. Robert A. Schulz, Nancy Fossett
        Pages 109-121
    3. Xenopus

    4. Zebrafish

      1. Noëlle N. Paffett-Lugassy, Leonard I. Zon
        Pages 171-198
    5. Chick

    6. Mouse

About this book

Introduction

Over the past decade technical and conceptual breakthroughs, cross-species comparisons, and multidisciplinary approaches have led to a virtual revolution in developmental biology. In Developmental Hematopoiesis: Methods and Protocols, a panel of expert investigators critically surveys an array of methods available to study hematopoiesis and describes in detail the powerful techniques they have established to investigate embryonic, fetal, and (to a lesser extent) postnatal blood development. The authors apply their approaches to invertebrate (fruit flies) as well as vertebrate (frog, fish, mouse, chick, and human) organisms. These state-of-the-art techniques range from the genetic, molecular, and cellular, to cell and embryo explant culture and whole animals, including in vivo imaging. Bioinformatic and functional genomic approaches for studying stem cells and their supportive stromal cells are also discussed. The protocols follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Developmental Hematopoiesis: Methods and Protocols offers novice and seasoned researchers alike the critical tools needed to gain a fundamental understanding of stem cell biology-examining not only how hematopoietic stem cells form, but also how they differ in the embryo, the fetus, and the adult.

Keywords

blood hematopoietic stem cell stem cells transplantation

Editors and affiliations

  • Margaret H. Baron
    • 1
  1. 1.Department of Medicine (Hematology-Oncology)Mount Sinai School of MedicineNew York

Bibliographic information

  • DOI https://doi.org/10.1385/1592598269
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-296-4
  • Online ISBN 978-1-59259-826-7
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
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