Abstract
In vivo experimental approaches that have been designed to study the ontogeny of the hematopoietic system in higher vertebrates are described in the present chapter. The avian embryo is directly available to manipulations in ovo during gastrulation and organogenesis. This permissiveness has led to the design of various approaches that provided crucial insights into the ontogeny of the hematopoietic system, particularly regarding traffic of progenitors between different compartments. In contrast, experimental manipulation of the developing mouse in utero is possible only during the second half of gestation, that is, the fetal period. This approach has been very useful in understanding how the immune system learns to distinguish self from nonself.
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Le Douarin, N. (1973) A biological cell labelling technique and its use in experimental Embryology. Dev. Biol. 30, 217–222.
Dieterlen-Lièvre, F., Pardanaud, L., Caprioli, A., and Jaffredo T. (2001) Non-yolk sac hematopoietic stem cells: the avian paradigm, in Hematopoiesis: A Developmental Approach (Zon, L., ed.), Oxford University Press, New York, NY, pp. 201–208.
Le Douarin, N. M., Corbel, C., Martin, C., Coltey, M., and Salaün, J. (1989) Induction of tolerance by embryonic thymic epithelial grafts in birds and mammals in Cold Spring Harbor Symposia on Quantitative Biology, vol. 65, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 777–787.
Le Douarin, N. M., Corbel, C., Bandeira, A., Thomas-Vaslin, V., Modigliani, Y., Coutinho, A., et al. (1996) Evidence for a thymus dependent form of tolerance that is not based on elimination or reactive T cells. Immunol. Rev. 149, 35–53.
Le Douarin, N. M., Dieterlen-Lièvre, F., and Teillet, M-A. (1996) Quail-chick transplantations. Methods Cell Biol. 51, 23–61.
Le Douarin, N. M., Dieterlen-Lièvre, F., Teillet, M-A., and Ziller, C. (2000) Interspecific chimeras in avian embryos. Methods Mol. Biol. 135, 373–386.
Hamburger, V. and Hamilton, H. L. (1951) A series of normal stages in the development of the chick embryo. J. Morphol. 88, 49–92.
Zacchei, A. M. (1961) Lo sviluppo embrionale della quaglia giapponese (Coturnix cotunix japonica). Arch. Ital. Anat. Embriol. 66, 36–62.
Metcalf, D. and Moore, M. A. S. (1971) Embryonic aspects of haemopoiesis, in Haemopoietic Cells (Neuberger, G., and Tatum, E. L., eds.), North Holland Publications, Amderdam, The Netherlands, pp. 127.
Le Douarin, N. M., Dieterlen-Lièvre, F., and Oliver, P. (1984) Ontogeny of primary lymphoid organs and lymphoid stem cells. Am. J. Anat. 170, 261–299.
Pardanaud, L., Altmann, C., Kitos, P., Dieterlen-Lièvre, F., and Buck, C. A. (1987) Vasculogenesis in the early quail blastodisc as studied with a monoclonal antibody recognizing endothelial cells. Development 100, 339–349.
Pardanaud, L., Yassine, F., and Dieterlen-Lièvre, F. (1989) Relationship between vasculogenesis, angiogenesis and haemopoiesis during avian ontogeny. Development 105, 473–485.
Martin, C. S. (1983) Total thymectomy in the early chick embryo. Arch. Anat. Micr. 72, 107–110.
Belo, M., Martin, C., Corbel, C., and Le Douarin, N. M. (1985) A novel method to bursectomize avian embryos and obtain quail/chick bursal chimeras I. Immunocytochemical analysis of such chimeras by using species-specific monoclonal antibodies. J. Immunol. 135, 3785–3794.
Dieterlen-Lièvre, F., Pardanaud, L., Bollérot, K., and Jaffredo, T. (2001) Hemangioblasts and hemopoietic stem cells during ontogeny. C. R. Acad. Sci. 325, 1013–1020.
Martin, C. (1972) Technique d’explantation in ovo de blastodermes d’embryons d’oiseaux. C. R. Séances Soc. Biol. ses Fil. 116, 283–285.
Martin, C., Beaupain, D., and Dieterlen-Lièvre, F. (1980) A study of the development of the hemopoietic system using quail-chick chimeras obtained by blastoderm recombination. Dev. Biol. 75, 303–314.
Muramatsu, T., Mizutani, Y., Ohmori, Y., and Okumura, I. (1997) Comparison of three non-viral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochem. Biophys. Res. Commun. 230, 376–380.
Momose, T., Tonegawa, A., Takeuchi, J., Ogawa, H., Umesono, K., and Yasuda, K. (1999) Efficient targeting of gene expression in chick embryos by microelectroporation. Dev. Growth Differ. 41, 335–344.
Mikawa, T., Fischman, D. A., Dougherty, J. P., and Brown, A. M. C. (1991). In vivo analysis of a new lacZ retrovirus vector suitable for cell lineage marking in avian and other species. Exp. Cell Res. 195, 516–523.
Grammatopoulos, G. A., Bell, E., Toole, L., Lumsden, A., and Tucker, A. S. (2000) Homeotic transformation of branchial arch identity after Hoxa2 overexpression. Development 127, 5355–5565.
Jaffredo, T., Gautier, R., Eichmann, A., and Dieterlen-Lièvre, F. (1998) Intraaortic hemopoietic cells are derived from endothelial cells during ontogeny. Development 125, 4575–4583.
Jaffredo, T., Gautier, R., Brajeul, V., and Dieterlen-Lièvre, F. (2000) Tracing the progeny of the aortic hemangioblast in the avian embryo. Dev. Biol. 224, 204–214.
Fleischman, R. A. and Mintz, B. (1979) Prevention of genetic anemias in mice by microinjection of normal hematopoietic stem cells into the fetal placenta. Proc. Natl. Acad. Sci. USA 76, 5736–5740.
Salaün, J., Calman, F., Coltey, M., and Le Douarin, N. M. (1986) Construction of chimeric thymuses in the mouse fetus by in utero surgery. Eur. J. Immunol. 16, 523–530.
Coutinho, A., Salaün, J., Corbel, C., Bandeira, A., and Le Douarin, N. M. (1993) The role of thymic epithelium in the establishment of transplantation tolerance. Immunol. Rev. 133, 225–240.
Till, J. E. and McCulloch, E. A. (1961) A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat. Res. 104, 213–222.
Salaün, J., Bandeira, A., Khazaal, I., Calman, F., Coltey, M., Coutinho, A., et al. (1990) Thymic epithelium tolerizes for histocompatibility antigens. Science 247, 1471–1474.
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Dieterlen-Lièver, F., Creuzet, S., Salaün, J. (2005). In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo. In: Baron, M.H. (eds) Developmental Hematopoiesis. Methods in Molecular Medicine, vol 105. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-826-9:215
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DOI: https://doi.org/10.1385/1-59259-826-9:215
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