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Developmental Hematopoiesis pp 215–229Cite as

In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 105))

Abstract

In vivo experimental approaches that have been designed to study the ontogeny of the hematopoietic system in higher vertebrates are described in the present chapter. The avian embryo is directly available to manipulations in ovo during gastrulation and organogenesis. This permissiveness has led to the design of various approaches that provided crucial insights into the ontogeny of the hematopoietic system, particularly regarding traffic of progenitors between different compartments. In contrast, experimental manipulation of the developing mouse in utero is possible only during the second half of gestation, that is, the fetal period. This approach has been very useful in understanding how the immune system learns to distinguish self from nonself.

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Authors and Affiliations

  1. Laboratoire d’Embryologie cellulaire et moléculaire du CNRS et Collège de France, Nogent-sur-Marne cedex, France

    Françoise Dieterlen-Lièver, Sophie Creuzet & Josselyne Salaün

Authors
  1. Françoise Dieterlen-Lièver
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  2. Sophie Creuzet
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  3. Josselyne Salaün
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Editors and Affiliations

  1. Department of Medicine (Hematology-Oncology), Mount Sinai School of Medicine, New York, NY

    Margaret H. Baron MD, PhD

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© 2005 Humana Press Inc., Totowa, NJ

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Dieterlen-Lièver, F., Creuzet, S., Salaün, J. (2005). In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo. In: Baron, M.H. (eds) Developmental Hematopoiesis. Methods in Molecular Medicine, vol 105. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-826-9:215

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  • DOI: https://doi.org/10.1385/1-59259-826-9:215

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-58829-296-4

  • Online ISBN: 978-1-59259-826-7

  • eBook Packages: Springer Protocols

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