Abstract
Because zebrafish embryos are transparent, cell behaviors and interactions can be directly imaged noninvasely in live embryos using differential interference contrast-Nomarski light microscopy. We found that the imaging quality can be much improved by coupling differential interference contrast-Nomarski to true (analogical) color video so as to visualize the image in real time on a high-resolution colour video monitor. We explain here how to apply this approach to the in vivo imaging of embryonic macrophages, which constitute a distinct early macrophage lineage, that originates from the rostral-most lateral mesoderm -adjacent to the cardiac field, differentiate in the yolk sac, and rapidly spread in embryonic tissues, although still retaining proliferative capacity.
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© 2005 Humana Press Inc., Totowa, NJ
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Herbomel, P., Levraud, JP. (2005). Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos. In: Baron, M.H. (eds) Developmental Hematopoiesis. Methods in Molecular Medicine, vol 105. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-826-9:199
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DOI: https://doi.org/10.1385/1-59259-826-9:199
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-58829-296-4
Online ISBN: 978-1-59259-826-7
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