Overview
- Editors:
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Udo Reischl
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University of Regensburg, Germany
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Table of contents (36 protocols)
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Recombinant Proteins and Peptide
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Purification of Recombinant Proteins
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- Raffaele A. Calogero, Anna Aulicino
Pages 345-360
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Evalution of Recombinant Proteins in Immunological Test Systems
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- Michael Kirschfink, Peter Terness
Pages 361-371
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- Richard Decker, David Leahy
Pages 397-410
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Antibodies
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Front Matter
Pages 485-485
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- Peter Appleby, Udo Reischl
Pages 487-502
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Biosensor Technology
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- Franz Aberl, Conrad Kößlinger
Pages 503-517
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- Franz Aberl, Conrad Kößlinger, Hans Wolf
Pages 519-529
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- Uwe W. Roder, Francis Markey
Pages 531-554
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Recombinant Antigens
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- Melvyn Little, Stefan Dübel, Sergey Kipriyanov, Frank Breitling
Pages 555-580
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- Frank Breitling, Stefan Dübel
Pages 581-591
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- Martin Welschof, Melvyn Little, Heinz Dörsam
Pages 593-603
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- Heinz Dörsam, Michael Braunagel, Christian Kleist, Daniel Moynet, Martin Welschof
Pages 605-614
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Back Matter
Pages 623-629
About this book
In most societies the medical field is undergoing a dramatic reorien- tion. Fundamentally new technologies in diagnosis and therapy, as well as the extension of life expectations, have increased health costs to an extent that now nears the limits of acceptability. One consequence has been a reevaluation of the need for and duration of each individual hospitalization. For diagnostic laboratories, results must now be obtained rapidly in order to include them in therapeutic decisions. Furth- more, therapeutic approaches to the control of infectious agents, limited by the number of therapeutic compounds available and by the growing perce- age of multiresistant agents, now require improvement in the precision of diagnostic approaches. The necessity for maximal reduction of the risk of infection by the development of appropriate pharmaceutical products demands even higher levels of sensitivity in diagnostic tests. Today's highly purified antigens---often from recombinantly generated antigens or peptides, monoclonal or recombinant antibodies, and more sen- tive signal-production and signal-detection systems--have greatly advanced immunologically based detection methodology. However, this approach has biological limits that further enhancement of sensitivity cannot breach. Fortunately nucleic acids have now been quite successfully added to the sp- trum of diagnostic targets. Recombinant-produced antigens and the integration of microelectronics and microfluidics offer new perspectives and possibilities.
Reviews
Review of the first edition:
"This work represents one of the most up-to-date molecular biology protocols books to date. . .Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book. . a valuable purchase to keep as a quick protocol source."-Archives of Pathology and Laboratory Medicine
Editors and Affiliations
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University of Regensburg, Germany
Udo Reischl