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Screening of Phage-Displayed Antibody Libraries

  • Protocol
Molecular Diagnosis of Infectious Diseases

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 13))

Abstract

The problem of amplifying a specific antibody in a population of millions of other antibodies has been solved by the immune system using the process of clonal selection Binding of an antigen to an IgM receptor on the surface of B-lymphocytes stimulates the proliferation and differentiation of the lymphocyte until it matures to an IgG-producing plasma cell. To mimic the first step of this process in bacteria, vectors have been constructed for the expression of antibodies on the surface of bacteria and phages (for review see Chapter 32)

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References

  1. Breitling F., Dubel S., Seehaus T., Klewinghaus I., and Little M (1991) A surface expression vector for antibody screening. Gene 104, 147–153

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Author information

Authors and Affiliations

  1. Recombinant Antibody Group, German Cancer Research Center, Heidelberg, Germany

    Heinz Dörsam & Michael Braunagel

  2. Department of Transplant Immunology, University of Heidelberg, Germany

    Christian Kleist

  3. INSERM, Bordeaux, France

    Daniel Moynet

  4. Department of Transplantation Immunology, University of Heidelberg, Germany

    Martin Welschof

Authors
  1. Heinz Dörsam
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  2. Michael Braunagel
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  3. Christian Kleist
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  4. Daniel Moynet
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  5. Martin Welschof
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Editor information

Editors and Affiliations

  1. University of Regensburg, Germany

    Udo Reischl

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© 1998 Humana Press Inc.

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Dörsam, H., Braunagel, M., Kleist, C., Moynet, D., Welschof, M. (1998). Screening of Phage-Displayed Antibody Libraries. In: Reischl, U. (eds) Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine™, vol 13. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-485-2:605

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  • DOI: https://doi.org/10.1385/0-89603-485-2:605

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-485-3

  • Online ISBN: 978-1-59259-597-6

  • eBook Packages: Springer Protocols

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