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Species Diagnostics Protocols

PCR and Other Nucleic acid Methods

  • Justin P. Clapp

Part of the Methods in Molecular Biology™ book series (MIMB, volume 50)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mien T. G. van de Ven, Patrick G. Lanham, Rex M. Brennan
    Pages 1-14
  3. Andrew F. Cockburn, Gary A. Fritz
    Pages 15-23
  4. David T. Bilton, Maarit Jaarola
    Pages 25-37
  5. Donato Gallitelli, Pasquale Saldarelli
    Pages 57-79
  6. Angelantonio Minafra, Donato Gallitelli
    Pages 81-91
  7. Jürg Böni
    Pages 93-107
  8. Kouichi Morita
    Pages 127-132
  9. Marco Bazzicalupo, Renato Fani
    Pages 155-175
  10. Monique Gardes, Thomas D. Bruns
    Pages 177-186
  11. Kesara Anamthawat-Jónsson, J. S. Pat Heslop-Harrison
    Pages 209-225
  12. Guillaume J. J. M. Van Eys, Stefanie E. O. Meredith
    Pages 227-242
  13. Richard L. Roehrdanx
    Pages 381-390
  14. Back Matter
    Pages 411-416

About this book

Introduction

Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods is premised on the rapid development in recent years of the use of nucleic acid-based technology for the identification of various organisms. The majority of applications have been in such medically related fields as the diagnosis of disease or the identification of dis­ ease vectors, but this emphasis is changing as the potential and range of the techniques becomes more generally recognized. This has been especially true since the advent of polymerase chain reaction (PCR) technology. The use of nucleic acids for diagnostic purposes offers numerous advantages over conventional methods of identification. It must be stressed, however, that conventional methods are still extremely valuable and will always remain at the forefront of many diagnostic procedures. The ability of the skilled taxonomist begins to fail when there is a requirement to identify rapidly organisms that are small, immature, or present in large numbers. In such cases the time scale necessary to obtain an identification often precludes practical usage. It is in this context that molecular methods come into their own. There are several advantages that DNA can offer diagnostics other than the obvious and important fact that its base sequence is the fundamental source of biological variation. It offers a great amount of information that is not accessible to other methods of identifica­ tion because the vast majority of DNA is not expressed.

Editors and affiliations

  • Justin P. Clapp
    • 1
  1. 1.Department of BiologyUniversity of YorkUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896033236
  • Copyright Information Humana Press 1996
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-323-8
  • Online ISBN 978-1-59259-537-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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