Abstract
Knowledge of the nucleotide sequence of a viral genome enables the design of specific oligonucleotides for use as primers for selective amplification of a target nucleic acid from a pool of complex template by a polymerase chain reaction (PCR) driven by Taq, a thermostable DNA polymerase (1,2). The amplified fragment can be analyzed by gel electrophoresis for its presence or characterized by nucleic acid hybridization and restriction enzyme digestion for its heterogeneity.
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© 1996 Humana Press Inc.
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Minafra, A., Gallitelli, D. (1996). Improved PCR Methods for Identification of Phytopathogenic Viruses. In: Clapp, J.P. (eds) Species Diagnostics Protocols. Methods in Molecular Biology™, vol 50. Humana Press. https://doi.org/10.1385/0-89603-323-6:81
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DOI: https://doi.org/10.1385/0-89603-323-6:81
Publisher Name: Humana Press
Print ISBN: 978-0-89603-323-8
Online ISBN: 978-1-59259-537-2
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