Abstract
Questions regarding species identification, differentiation, and relationships often emerge in biological investigations. One may need to examine the extent of genetic relationships between closely related species for the purposes of resource management or assessment of biodiversity. It may also be important to verify the identity of commercial clones and cultivars used in agriculture and forestry, to detect pathological diseases, or to evaluate evolutionary changes at the molecular and organismic levels. Since interspecific and intergeneric hybridization forms an important part of evolution in many plant species, it is often necessary to determine the ancestors of polyploids, or the origin of alien chromosomes or chromosome segments in natural and cultivated hybrid derivatives.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Schwarzacher, T., Leitch, A. R., Bennett, M. D., and Heslop-Harrison, J. S. (1989) In situ localization of parental genomes in a wide hybrid. Ann. Bot. 64, 315–324.
Anamthawat-Jónsson, K., Schwarzacher, T., Leitch, A. R., Bennett, M. D., and Heslop-Harrison, J. S. (1990) Discrimination between closely related Triticeae species using genomic DNA as a probe. Theor. Appl. Genet. 79, 721–728.
Heslop-Hanson, J. S., Leitch, A. R., Schwarzacher, T., and Anamthawat-Jónsson, K. (1990) Detection and characterization of 1B/1R translocations in hexaploid wheat. Heredity 65, 385–392.
Leitch, A. R., Schwarzacher, T., Mosgoller, W., Bennett, M. D., and Heslop-Harrison, J. S. (1991) Parental genomes are separated throughout the cell cycle in a plant hybrid. Chromosoma 101, 206–213.
Schwarzacher, T., Anamthawat-Jónsson, K., Harrison, G. E., Islam, A. K. M. R., Jia, J. Z., King, I. P., Leitch, A. R., Miller, T. E., Reader, S. M., Rogers, W. J., Shi, M., and Heslop-Harrison, J. S. (1992) Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat. Theor. Appl. Genet. 84, 778–786.
Anamthawat-Jónsson, K., Schwarzacher, T., and Heslop-Harrison, J. S. (1993) Behavior of parental genomes in the hybrid Hordeum vulgare × H. bulbosum. J. Heredity 84, 78–82.
Heslop-Harrison, J. S. and Schwarzacher, T. (1993) Molecular cytogenetics—biology and applications in plant breeding, in Chromosomes Today, vol. 11 (Sumner, A. T. and Chandley, A. C., eds.), Chapman and Hall, London, pp. 191–198.
Yap, K. W., Thompson, R. C. A., and Pawlowski, I. D. (1989) The development of nonradioactive total genomic probes for the strain and egg differentiation in taeniid cestodes. Am. J. Trop. Med. Hyg. 39, 472–477.
Hopper, B. R., Sanborn, M. R, and Bantle, J. A. (1989) Detection of Brucella abortus in mammalian tissue, using biotinylated, whole genomic DNA as a molecular probe. Am. J. Vet. Res. 50, 2064–2068.
Pinkel, D., Straume, T., and Gray, J. W. (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc. Natl. Acad. Sci. USA 83, 2934–2938.
Kallioniemi, A., Kallioniemi, O. P., Sudar, D., Rutovitz, D., Gray, J. W., Waldman, F., and Pinkel, D. (1992) Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 258, 818–821.
Anamthawat-Jónsson, K. and Heslop-Harrison, J. S. (1992) Species specific DNA sequences in the Triticeae Hereditas. 116, 49–54.
Anamthawat-Jónsson, K. and Heslop-Harrison, J. S. (1993) Isolation and characterization of genome-specific DNA sequences in Triticeae species. Mol. Gen. Genet. 240, 151–158.
Eyfjord, J. E., Thorlacius, S., Steinarsdóttir, M., Valgarosdóttir, R., Ogmundsdóttir, H. M., and Anamthawat-Jónsson, K. (1994) P53 abnormalities and genomic instability in primary human breast carcinomas. Cancer Res. 55, 646–651.
Ørgaard, M. and Heslop-Harrison, J. S. (1994) Investigations of genome relationships between Leymus, Psathyrostachys, and Hordeum inferred by genomic DNA:DNA in situ hybridization. Ann. Bot. 73, 195–203.
Schwarzacher, T. and Leitch, A. R. (1994) Enzymatic treatment of plant material to spread chromosomes for in situ hybridization, in Methods in Molecular Biology, Vol 28: Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Isaac, P. G., ed.), Humana, Totowa, NJ, pp. 153–160.
Ørgaard, M. and Heslop-Harrison, J. S. (1994) Relationships between species of Leymus, Psathyrostachys, and Hordeum (Poaceae, Triticeae) inferred from Southern hybridization of genomic and cloned DNA probes. Plant Syst. Evol. 189, 217–231.
Meinkoth, J. and Wahl, G. (1984) Hybridization of nucleic acids immobilized on solid supports. Anal. Biochem. 138, 267–284.
Karp, A. (1994) Use of biotin-labeled probes on plant chromosomes, in Methods in Molecular Biology, Vol. 28: Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Isaac, P. G., ed.), Humana, Totowa, NJ, pp. 161–166.
Schwarzacher, T. and Heslop-Harrison, J. S. (1994) Direct fluorochrome-labeled DNA probes for direct fluorescent in situ hybridization to chromosomes, in Methods in Molecular Biology, Vol 28: Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Isaac, P. G., ed.), Humana, Totowa, NJ, pp. 167–176.
Leitch, I. J. and Heslop-Harrison, J. S. (1994) Detection of digoxigenin-labeled DNA probes hybridized to plant chromosomes in situ, in Methods in Molecular Biology, Vol. 28: Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Isaac, P. G., ed.), Humana, Totowa, NJ, pp. 177–185.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Humana Press Inc.
About this protocol
Cite this protocol
Anamthawat-Jónsson, K., Heslop-Harrison, J.S.P. (1996). Establishing Relationships Between Closely Related Species Using Total Genomic DNA as a Probe. In: Clapp, J.P. (eds) Species Diagnostics Protocols. Methods in Molecular Biology™, vol 50. Humana Press. https://doi.org/10.1385/0-89603-323-6:209
Download citation
DOI: https://doi.org/10.1385/0-89603-323-6:209
Publisher Name: Humana Press
Print ISBN: 978-0-89603-323-8
Online ISBN: 978-1-59259-537-2
eBook Packages: Springer Protocols