Vertebrate Embryogenesis

Embryological, Cellular, and Genetic Methods

  • Francisco J. Pelegri

Part of the Methods in Molecular Biology book series (MIMB, volume 770)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mizuho S. Mimoto, Jan L. Christian
    Pages 55-75
  3. Timothy J. Geach, Lyle B. Zimmerman
    Pages 77-117
  4. Laura S. Gammill, Catherine E. Krull
    Pages 119-137
  5. Yuhua Sun, Dorota Wloga, Scott T. Dougan
    Pages 139-184
  6. Sreelaja Nair, Francisco J. Pelegri
    Pages 185-209
  7. Anna Piliszek, Gloria S. Kwon, Anna-Katerina Hadjantonakis
    Pages 243-257
  8. Edwina McGlinn, Jennifer H. Mansfield
    Pages 259-292
  9. Anne E. Griep, Manorama C. John, Sakae Ikeda, Akihiro Ikeda
    Pages 293-312
  10. Vanessa L. Horner, Tamara Caspary
    Pages 313-336
  11. Tien-cheng Arthur Chang, Anthony W. S. Chan
    Pages 337-363
  12. Christian Sardet, Alex McDougall, Hitoyoshi Yasuo, Janet Chenevert, Gérard Pruliere, Rémi Dumollard et al.
    Pages 365-400
  13. Michael T. Veeman, Shota Chiba, William C. Smith
    Pages 401-422
  14. Matthew K. Vickaryous, Katherine E. McLean
    Pages 439-455
  15. Sylke Winkler, Nicola Gscheidel, Michael Brand
    Pages 475-504

About this book

Introduction

One of the striking findings of modern developmental biology has been the high degree of conservation of signaling and developmental mechanisms amongst different animal species. Such conservation allows information learned from a given organism to be applicable to other species, including humans, and has validated the use of a few model systems to deduce general biological principles. In spite of this underlying conservation, however, each species has unique characteristics arising from its evolutionary history. Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species. In fact, this volume has been designed so that readers can readily find information on species other than the one with which they may be most familiar. These protocols include not only embryological methods, but also cellular and genetic approaches that have complemented and expanded our understanding of embryonic development.  In addition, a number of chapters highlight a specific method that is in principle applicable to multiple species, such as TILLING and ZFN-mediated mutagenesis, the generation of Embryonic Stem (ES) cell lines, and nuclear/oocyte transfer.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods serves as an ideal guide to the molecular, cell, and developmental biology community and will hopefully contribute to the ongoing collective effort towards a better understanding of the beauty and logic of vertebrate development.

Keywords

TILLING ZFN-mediated mutagenesis medaka mouse embryos somatic cells xenopus laevis zebrafish

Editors and affiliations

  • Francisco J. Pelegri
    • 1
  1. 1.Dept. GeneticsUniversity of Wisconsin, MadisonMadisonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-210-6
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-209-0
  • Online ISBN 978-1-61779-210-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Biotechnology
Consumer Packaged Goods
Pharma