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Ciona Genetics

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Vertebrate Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 770))

Abstract

Ascidians, such as Ciona, are invertebrate chordates with simple embryonic body plans and small, relatively non-redundant genomes. Ciona genetics is in its infancy compared to many other model systems, but it provides a powerful method for studying this important vertebrate outgroup. Here we give basic methods for genetic analysis of Ciona, including protocols for controlled crosses both by natural spawning and by the surgical isolation of gametes; the identification and propagation of mutant lines; and strategies for positional cloning.

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Author information

Authors and Affiliations

  1. Department of Molecular, Cell and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA, USA

    Michael T. Veeman, Shota Chiba & William C. Smith

Authors
  1. Michael T. Veeman
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  2. Shota Chiba
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  3. William C. Smith
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Corresponding author

Correspondence to William C. Smith .

Editor information

Editors and Affiliations

  1. Dept. Genetics, University of Wisconsin, Madison, Henry Mall 425-G, Madison, 53706-1574, Wisconsin, USA

    Francisco J. Pelegri

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© 2011 Springer Science+Business Media, LLC

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Veeman, M.T., Chiba, S., Smith, W.C. (2011). Ciona Genetics. In: Pelegri, F. (eds) Vertebrate Embryogenesis. Methods in Molecular Biology, vol 770. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-210-6_15

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  • DOI: https://doi.org/10.1007/978-1-61779-210-6_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-209-0

  • Online ISBN: 978-1-61779-210-6

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