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Embryological Manipulations in Zebrafish

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Vertebrate Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 770))

Abstract

Due to the powerful combination of genetic and embryological techniques, the teleost fish Danio rerio has emerged in the last decade as an important model organism for the study of embryonic development. It is relatively easy to inject material such as mRNA or synthetic oligonucleotides to reduce or increase the expression of a gene product. Changes in gene expression can be analyzed at the level of mRNA, by whole-mount in situ hybridization, or at the level of protein, by immunofluorescence. It is also possible to quantitatively analyze protein levels by Western and immunoprecipitation. Cell behavior can be analyzed in detail by cell transplantation and by fate mapping. Because a large number of mutations have been identified in recent years, these methods can be applied in a variety of contexts to provide a deep understanding of gene function that is often more difficult to achieve in other vertebrate model systems.

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Acknowledgments

We thank Ben Feldman for providing Kaede protein, Joeseph Frankel for providing the 12G10 antibody (available from the Developmental Studies Hybridoma Bank, developed under the auspices of NICHD and maintained by the University of Iowa), Jim Lauderdale for advice on the protocol for WISH followed by immunohistochemistry, and Rebecca Ball for helpful comments on the manuscript. S.T.D. is a Georgia Cancer Coalition distinguished investigator. Work in the Dougan lab is supported by the American Cancer Society (RSGDDC-112979).

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Authors and Affiliations

  1. Department of Cellular Biology, The University of Georgia, Athens, GA, USA

    Yuhua Sun & Scott T. Dougan

  2. Nencki Institute of Experimental Biology, Polish Academy of Science, Warsaw, Poland

    Dorota Wloga

Authors
  1. Yuhua Sun
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  2. Dorota Wloga
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  3. Scott T. Dougan
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Corresponding author

Correspondence to Scott T. Dougan .

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Editors and Affiliations

  1. Dept. Genetics, University of Wisconsin, Madison, Henry Mall 425-G, Madison, 53706-1574, Wisconsin, USA

    Francisco J. Pelegri

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Sun, Y., Wloga, D., Dougan, S.T. (2011). Embryological Manipulations in Zebrafish. In: Pelegri, F. (eds) Vertebrate Embryogenesis. Methods in Molecular Biology, vol 770. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-210-6_6

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  • DOI: https://doi.org/10.1007/978-1-61779-210-6_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-209-0

  • Online ISBN: 978-1-61779-210-6

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