Helicases

Methods and Protocols

  • Mohamed M. Abdelhaleem

Part of the Methods in Molecular Biology book series (MIMB, volume 587)

Table of contents

  1. Front Matter
    Pages i-x
  2. Mohamed Abdelhaleem
    Pages 1-12
  3. Eli Rothenberg, Taekjip Ha
    Pages 29-43
  4. Christopher J. Fischer, Lake Wooten, Eric J. Tomko, Timothy M. Lohman
    Pages 45-56
  5. Manjula Pandey, Mikhail K. Levin, Smita S. Patel
    Pages 57-83
  6. Laxmi Yeruva, Kevin D. Raney
    Pages 85-98
  7. Matthew Jessulat, Terry Buist, Md Alamgir, Mohsen Hooshyar, Jianhua Xu, Hiroyuki Aoki et al.
    Pages 99-111
  8. Daniel L. Kaplan, Irina Bruck
    Pages 127-135
  9. Marc Boudvillain, Céline Walmacq, Annie Schwartz, Frédérique Jacquinot
    Pages 137-154
  10. Thomas T Perkins, Hung-Wen Li
    Pages 155-172
  11. Xu Guang Xi
    Pages 173-184
  12. Daniela Kobbe, Manfred Focke, Holger Puchta
    Pages 195-209
  13. Mariusz Krawczyk, Anna Stankiewicz-Drogoń, Anne-Lise Haenni, Anna Boguszewska-Chachulska
    Pages 211-221
  14. David N Frick, Olya Ginzburg, Angela M.I Lam
    Pages 223-233
  15. Eckhard Jankowsky, Andrea Putnam
    Pages 245-264
  16. Samantha M. Nicol, Frances V. Fuller-Pace
    Pages 265-279

About this book

Introduction

In recent years, much information has been revealed concerning the essential role of helicases, the enzymes that utilize the energy derived from nucleoside triphosphate hydrolysis to unwind the double stranded helical structure of nucleic acids. In Helicases: Methods and Protocols, this topic is explored by leading international scientists who have contributed significantly to our current understanding of helicases and the ways in which they function. Chapters include methods for the production and purification of helicases from different species, as well as detailed studies of helicase activities, including NTP binding and hydrolysis, nucleic acid binding and unwinding, and translocation along nucleic acid substrates. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and innovative, Helicases: Methods and Protocols is the ultimate guide for researchers, providing the scientific community with comprehensive accounts of the most advanced methods used to study helicases today.

Keywords

DNA Escherichia coli RNA Termination genes molecular biology saccharomyces cerevisiae virus

Editors and affiliations

  • Mohamed M. Abdelhaleem
    • 1
  1. 1.Dept. Paediatric Laboratory MedicineHospital for Sick ChildrenTorontoCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-355-8
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-354-1
  • Online ISBN 978-1-60327-355-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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